文献类型：期刊文献

英文题名：Revealing the composition and mechanism of the antibacterial activity of essential oil from the fruits of Litsea mollis Hemsl

作者：Feng, S. Y.[1] Chen, D.[2] Chen, Y. C.[3,4] Wang, Y. D.[3,4] Song, D. F.[1] Gao, M.[3,4] Gu, Q.[1]

第一作者：Feng, S. Y.

通信作者：Song, DF[1]

机构：[1]Zhejiang Gongshang Univ, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou 310018, Peoples R China;[2]Hangzhou Anxu Biotech Co Ltd, Hangzhou 310000, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100000, Peoples R China;[4]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou 310012, Peoples R China

年份：2023

卷号：30

期号：1

起止页码：79-86

外文期刊名：INTERNATIONAL FOOD RESEARCH JOURNAL

收录：;Scopus(收录号：2-s2.0-85149652434);WOS:【SCI-EXPANDED(收录号:WOS:001054027600002)】；

基金：The present work was financially supported by the Natural Science Foundation of Zhejiang Province (grant no.: LY18C060004).

语种：英文

外文关键词：Litsea mollis Hemsl; essential oil no. 2; chemical composition; antibacterial activity; antibacterial mechanism; citral

摘要：Essential oils (EOs) have received increasing attention due to their safety and effective antibacterial activity. The chemical components and antibacterial activity of Litsea mollis Hemsl. essential oil no. 2 (LMEO2) was investigated in the present work. GC- MS analysis was performed, and 24 bioactive compounds were detected with citral being the most predominant (46.1482%). LMEO2 had high antibacterial activity against the foodborne pathogens Escherichia coli O157 and Salmonella Enteritidis. The minimum inhibitory concentration (MIC) was 0.01% for E. coli O157, and 0.05% for Salmonella Enteritidis, and the minimum bactericidal concentration (MBC) was 0.05% for E. coli O157, and 0.1% for Salmonella Enteritidis. Furthermore, the alkaline phosphatase (AKP) leak assays revealed that LMEO2 destroyed the integrity of cell wall. This was consistent with the nucleic acid leakage assays which revealed that LMEO2 enhanced the membrane permeability. Meanwhile, the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) assays also demonstrated that LMEO2 could damage the cell wall integrity, thus leading to cellular content leakage. The main bioactive component of LMEO2 was citral. LMEO2 could be a promising plant-derived antibacterial agent.