文献类型：期刊文献

中文题名：硒驱动香蕉抗氧化系统激活抵御枯萎病菌的机制

英文题名：Mechanistic insights into selenium?driven activation of antioxidant enzyme system in bananas against Fusarium wilt pathogen

作者：罗英[1,2] 丁月[1,2] 郑泗军[2] 刘坤华[2] 王成业[3] 尹可锁[2] 徐胜涛[2] 任禛[1] 李成云[4] 刘立娜[2]

第一作者：罗英

机构：[1]昆明学院,昆明650214;[2]云南省农业科学院农业环境资源研究所,云南省农业跨境有害生物绿色防控重点实验室,昆明650205;[3]中国林业科学研究院高原林业研究所,昆明650224;[4]云南农业大学,省部共建云南生物资源保护与利用国家重点实验室,昆明650201

年份：2025

卷号：44

期号：6

起止页码：1980-1989

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划(2023YFD1400800);云南省基础研究专项(202201AT070062);国家自然科学基金项目(NSFC32161143001);兴滇英才支持计划(XDYC-QNRC-2022-0217)资助。

语种：中文

中文关键词：硒元素;香蕉种质;枯萎病;抗氧化酶;谷胱甘肽过氧化物酶

外文关键词：selenium;banana germplasm;Fusarium wilt;antioxidant enzyme;GSH-Px

分类号：S436.68

摘要：尖孢镰刀菌古巴专化型4号生理小种热带型(Foc TR4)引起的枯萎病严重制约着香蕉产业的可持续发展。研究发现,硒对广泛种植的香蕉种质Baxi Jiao具有防控枯萎病的功能,然而其机制尚不清楚。本研究以5个香蕉种质(Mbwazirume、Inkira、GCTCV-119、Gros Michel和Banksii)为对象,通过施加40 mg·L^(-1)硒处理并接种Foc TR4,调查香蕉种质的病情指数变化,检测不同时间点样本组织的总硒含量和抗氧化系统的响应特征,包括超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和还原型谷胱甘肽(GSH)含量,旨在阐明硒诱导香蕉抵御枯萎病菌的生理生化机制。结果表明,硒添加显著降低3个种质(Mbwazirume、Inkira、GCTCV-119)的病情指数,防效达到41.7%~62.6%,须根、叶片硒含量和富集系数高于防效不显著的种质。硒添加提高了Mbwazirume和GCTCV-119的GSH-Px酶活性,维持或提高GSH含量,参与GSH-Px酶活性与GSH含量动态平衡的快速响应;病原菌接种后叶片中的POD酶活性先升高,后降低,再升高,分别于接种后第7天和第56天达到峰值,并维持正常范围值265.9~351.7μmol·min^(-1)·g^(-1)。硒添加也直接提高了PPO酶活性。本研究揭示了香蕉不同种质的硒富集能力与防控效果之间的相关性,以不同的方式调节寄主抗氧化酶(GSH-Px、POD和PPO)活性是硒诱导不同抗病效果的机制之一,研究结果为进一步利用硒元素防控香蕉枯萎病提供了依据。
Fusarium wilt disease,caused by the virulent tropical race 4 of Fusarium oxysporum f.sp.cubense(Foc TR4),poses a significant threat to the sustainable development of banana production industry.Selenium plays a crucial role in the prevention of Foc TR4 in the widely cultivated banana germplasm,Baxi Jiao.However,the un?derlying mechanisms remain unclear.The disease indices of five banana germplasms(Mbwazirume,Inkira,GCTCV?119,Gros Michel,and Banksii)were evaluated following treatment with 40 mg·L^(-1)of selenium and inoc?ulation with Foc TR4.We investigated the total selenium content,antioxidant enzyme activities[superoxide dis?mutase(SOD),peroxidase(POD),polyphenol oxidase(PPO),catalase(CAT),glutathione peroxidase(GSH?Px)],and glutathione(GSH)content in tissues at different time points to elucidate the mechanism of selenium?mediated resistance in bananas against Foc TR4.The results showed that selenium addition significantly reduced the disease index across the three germplasms(Mbwazirume,Inkira,and GCTCV?119),with controlling effects ran?ging from 41.7%to 62.6%.These germplasms had higher selenium content and enrichment coefficients in roots and leaves compared to germplasms with negligible control effects.Selenium enhanced GSH?Px enzyme activity in Mb?wazirume and GCTCV?119,either maintaining or increasing GSH content,and contributed to the dynamic equilibrium between GSH?Px activity and GSH content.The POD activity in leaves,after pathogen inoculation,exhibited an initial increase,followed by a decrease,and then a resurgence,reaching peak values on 7th and 56th day after inoculation,respectively.Throughout this process,the peak values of POD activity remained within the normal range of 265.9 to 351.7μmol·min^(-1)·g^(-1).Furthermore,selenium significantly enhanced the PPO activity directly.This study elucidates the relationship between selenium enrichment ability and the preventive effects across dif?ferent banana germplasms.The differential regulation of host antioxidant enzymes(GSHPx,POD,and PPO)may serve as a mechanism by which selenium enhances resistance to Foc TR4.These findings provide a foundation for further exploration of the role of selenium in combating banana wilt disease.