文献类型：期刊文献

英文题名：Transcriptional and post-transcriptional regulation of the miR171-LaSCL6 module during somatic embryogenesis in Larix kaempferi

作者：Zang, Qiao-Lu[1] Zhang, Yao[2] Han, Su-Ying[2] Li, Wan-Feng[1] Qi, Li-Wang[1]

第一作者：Zang, Qiao-Lu

通信作者：Li, Wan-Feng|[a00054da667e87ce3e096]李万峰;

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Environment and Protection, Research Institute of Forest Ecology, Chinese Academy of Forestry, Beijing, 100091, China

年份：2021

卷号：35

期号：1

起止页码：145-154

外文期刊名：Trees - Structure and Function

收录：EI(收录号：20203609143202);Scopus(收录号：2-s2.0-85090169086)

语种：英文

外文关键词：Animal cell culture - RNA - Plants (botany) - Lanthanum compounds

摘要：Key message: Expression analysis of Larix kaempferi mature miR171s and their primary transcripts and target gene LaSCL6 during somatic embryogenesis revealed the transcriptional and post-transcriptional regulation of the miR171-LaSCL6 module. Abstract: Somatic embryogenesis provides a useful experimental system for studying the regulatory mechanisms of plant development. The level and activity of microRNA171 (miR171) fluctuate during somatic embryogenesis in Larix kaempferi, but the underlying mechanisms are still unclear. Here, in L. kaempferi we identified five members of the miR171 family, which cleave LaSCL6 mRNA at different sites. In addition, we improved the method of measuring miRNA activity in a more direct way. Furthermore, we measured the expression patterns of mature miR171s and their primary transcripts during somatic embryogenesis in L. kaempferi and found that their patterns differed, indicating that the transcription of MIR171 genes and the subsequent cleavage of their intermediate products are regulated. Taken together, our findings not only offer a means to study the regulation of miRNA activity, but also provide further insight into the regulation of L. kaempferi somatic embryogenesis by miR171-LaSCL6. ? 2020, The Author(s).