文献类型：期刊文献

英文题名：The LEA2 gene sub-family: Characterization, evolution, and potential functions in Camellia oleifera seed development and stress response

作者：Liu, Linxiu[1,2,3] Xu, Jing[1,3] Chen, Juanjuan[1,3] Yang, Bingbing[1,3] Yang, Chaochen[1,3] Yang, Yuchen[1,3] Wang, Kailiang[1,3] Zhuo, Renying[1,3] Yao, Xiaohua[1,3]

第一作者：Liu, Linxiu

通信作者：Zhuo, RY[1];Yao, XH[1];Zhuo, RY[2];Yao, XH[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Fac Forestry, Nanjing 210000, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China

年份：2023

卷号：322

外文期刊名：SCIENTIA HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-85167438444);WOS:【SCI-EXPANDED(收录号:WOS:001059732000001)】；

基金：This research was supported by the National Key R&D Program of China (2019YFD1001602) and Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2017ZA004-1) .

语种：英文

外文关键词：Camellia oleifera; LEA gene family; Seed development; Stress response

摘要：Camellia oleifera (C. oleifera) is a woody plant that is important economically due to its oil production. Late embryonic abundant (LEA) proteins are hydrophilic and thermostable proteins that accumulate during the late stages of embryo development, serving vital functions in plant development, seed oil accumulation, and stress responses. However, it remains unclear how LEAs regulate seed size, oil accumulation, and stress responses in C. oleifera. In our study, 47 LEA genes were identified across the C. oleifera genome, which were named based on their chromosomal distribution and separated into LEA2 subgroups. Ancient duplication events resulted in 3 tandem and 8 segmental duplications in the C. oleifera genome. Further analysis of genetic structure, motifs, and cis-elements demonstrated that the evolutionary relationships of the LEA gene family in C. oleifera are highly conserved with many of the LEA genes located in the chloroplast. WGCNA was used to identify modules containing co-expressed genes in different seed developmental stages, and the regulatory network revealed that CoLEA6, CoLEA26, CoLEA17, and CoLEA22 were the hub genes, acting alongside the genes involved in plant growth and abiotic stress. The qPCR analysis of 8 C. oleifera LEA genes under simulated drought stress conditions and gradient shade was conducted, and indicated that CoLEAs responded to drought and shade stresses. Overall, CoLEA22 may be critical for responses to abiotic stress and seed development. This study enable valuable insights into the roles of LEA genes in C. oleifera and establishes a foundation for further research on their function in regulating seed development, oil accumulation and stress response.