文献类型：期刊文献

英文题名：Multi-omics analysis reveals the regulatory mechanism of branching development in Quercus fabri

作者：Xiong, Shifa[1,2] Wu, Liwen[1,2] Chen, Yicun[1,2] Shi, Xiang[2] Wang, Yangdong[1,2]

第一作者：Xiong, Shifa

通信作者：Wang, YD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2025

卷号：313

外文期刊名：JOURNAL OF PROTEOMICS

收录：;Scopus(收录号：2-s2.0-85214305087);WOS:【SCI-EXPANDED(收录号:WOS:001409265700001)】；

基金：This work was supported by grants from the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties (2021C02070-9) and National Key Research and Development Program of China (2021YFD2200302) .

语种：英文

外文关键词：Multi-omics; Branching development; AP2/ERF; Plant hormone; Q. Fabri

摘要：The ability of axillary meristems to form axillary buds and subsequently develop into branches is influenced by phytohormones, environmental conditions, and genetic factors. The main trunk of Quercus fabri is prone to branching, which not only impacts the appearance and density of the wood and significantly reduces the yield rate. This study conducted transcriptomic, proteomic, and metabolomic analyses on three stages of axillary bud development in Q. fabri. A total of 12,888 differentially expressed genes (DEGs), 8193 differentially accumulated proteins (DAPs), and 1788 differentially accumulated metabolites (DAMs) were identified through comparisons among the stages and subjected to multi-omics joint analysis. Conduct interaction network analysis on DEGs and DAPs to identify the significant transcription factor family (AP2/ERF) involved in the regulation of axillary bud development. Furthermore, KEGG enrichment analysis of DEGs, DAPs and DAMs indicated significant enrichment in plant hormone signaling pathways. The analysis of endogenous hormone levels and qRT-PCR results for pathway genes demonstrated that the expression levels of IAA and tZ significantly increased during late developmental stages, whereas the expression levels of ABA, ACC and JA significantly decreased. In summary, these findings contribute to a comprehensive understanding of the regulatory networks underlying the branching development of Q. fabri. Significance: Q. fabri exhibits robust vegetative growth, and its primary trunk is prone to branching, significantly influencing the wood yield rate. Through a joint analysis of transcriptomics, proteomics, and metabolomics, we comprehensively examined the regulatory network governing the axillary bud development of Q. fabri. Our findings revealed the crucial roles of the AP2/ERF transcription factor family and plant hormone signal transduction pathways in branch development. These insights contribute to a deeper understanding of the mechanisms regulating branch development.