文献类型：期刊文献

英文题名：Responses of GABA shunt coupled with carbon and nitrogen metabolism in poplar under NaCl and CdCl2 stresses

作者：Ji, Jing[1] Shi, Zheng[2] Xie, Tiantian[1] Zhang, Xiaoman[3] Chen, Wei[1] Du, Changjian[1] Sun, Jiacheng[1] Yue, Jianyun[1] Zhao, Xiulian[1] Jiang, Zeping[2] Shi, Shengqing[1]

通信作者：Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, 1958 Box, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Forest Ecol & Environm State Forestry & G, Res Inst Forest Ecol Environm & Protect, 1958 Box, Beijing 100091, Peoples R China;[3]Hebei Agr Univ, Coll Landscape Architecture & Tourism, 289 Lingyusi St, Baoding 071001, Hebei, Peoples R China

年份：2020

卷号：193

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000521519900010)】；

基金：This work was supported by the National Natural Science Foundation of China (31971627), the National Key Technology R&D Program during the Twelfth Five-year Plan Period (2015BAD07B0103), the Central Public-interest Scientific Institution Basal Research Fund (CAFYBB2017SY003; CAFYBB2014ZX001-3), and the National Key R&D Program of China (2016YFD0600105).

语种：英文

外文关键词：GABA shunt; Carbon and nitrogen metabolism; NaCl stress; CdCl2 stress; Poplar

摘要：The gamma-aminobutyric acid (GABA) shunt is closely associated with plant tolerance; however, little is known about its mechanism. This study aimed to decipher the responses of the GABA shunt and related carbon-nitrogen metabolism in poplar seedlings (Populus alba x Populus glandulosa) treated with different NaCl and CdCl2 concentrations for 30 h. The results showed that the activities of glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) were activated, as well as alpha-ketoglutarate dehydrogenase (alpha-KGDH) and succinate dehydrogenase (SDH) activities were enhanced by NaCl and CdCl2 stresses, except for SDH under CdCl2 stress. Meanwhile, the expression levels of GADs, GABA-Ts SDHs, succinyl-CoA ligases (SCSs), and succinic acid aldehyde dehydrogenases (SSADHs) were also increased. Notably, significant increases in the key components of GABA shunt, Glu and GABA, were observed under both stresses. Soluble sugars and free amino acids were enhanced, whereas citrate, malate and succinate were almost inhibited by both NaCl and CdCl2 stresses except that citrate was not changed or just increased by 50-mM NaCl stress. Thus, these results suggested that the carbon-nitrogen balance could be altered by activating the GABA shunt when main TCA-cycle intermediates were inhibited under NaCl and CdCl2 stresses. This study can enhance the understanding about the functions of the GABA shunt in woody plants under abiotic stresses and may be applied to the genetic improvement of trees for phytoremediation.