文献类型：期刊文献

英文题名：CIPK11: a calcineurin B-like protein-interacting protein kinase from Nitraria tangutorum, confers tolerance to salt and drought in Arabidopsis

作者：Lu, Lu[1,2] Chen, Xinying[1] Wang, Pengkai[1] Lu, Ye[1] Zhang, Jingbo[3] Yang, Xiuyan[4] Cheng, Tielong[1] Shi, Jisen[1] Chen, Jinhui[1]

第一作者：Lu, Lu

通信作者：Chen, JH[1]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou, Inner Mongolia, Peoples R China;[4]China Acad Forestry, Natl Forestry & Grassland Adm, Res Ctr Saline & Alkali Land, Beijing 100091, Peoples R China

年份：2021

卷号：21

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85101822184);WOS:【SCI-EXPANDED(收录号:WOS:000625386600002)】；

基金：This research was supported by the Nature Science Foundation of China (31770715), the Key Research and Development Plan of Jiangsu Province (BE2017376), the Foundation of Jiangsu Forestry Bureau (LYKJ [2017]42), the Qinglan Project of Jiangsu Province, the Natural Science Foundation of Jiangsu Province (BK20181176), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Doctorate Fellowship Foundation of Nanjing Forestry University (grant 163010107). The funding organizations did not play any role in the design of the study, data collection and analysis, interpretation of data, or writing the manuscript.

语种：英文

外文关键词：Halophyte; Nitraria tangutorum; CIPK11; Salt stress; Drought stress

摘要：BackgroundThe CIPKs are a group of plant-specific Ser/Thr protein kinases acting in response to calcium signaling, which plays an important role in the physiological and developmental adaptation of plants to adverse environments. However, the functions of halophyte-derived CIPKs are still poorly understood, that limits a potential application of CIPKs from halophytes for improving the tolerance of glycophytes to abiotic stresses.ResultsIn this study, we characterized the NtCIPK11 gene from the halophyte Nitraria tangutorum and subsequently analyzed its role in salt and drought stress tolerance, using Arabidopsis as a transgenic model system. NtCIPK11 expression was upregulated in N. tangutorum root, stem and blade tissues after salt or drought treatment. Overexpressing NtCIPK11 in Arabidopsis improved seed germination on medium containing different levels of NaCl. Moreover, the transgenic plants grew more vigorously under salt stress and developed longer roots under salt or drought conditions than the WT plants. Furthermore, NtCIPK11 overexpression altered the transcription of genes encoding key enzymes involved in proline metabolism in Arabidopsis exposed to salinity, however, which genes showed a relatively weak expression in the transgenic Arabidopsis undergoing mannitol treatment, a situation that mimics drought stress. Besides, the proline significantly accumulated in NtCIPK11-overexpressing plants compared with WT under NaCl treatment, but that was not observed in the transgenic plants under drought stress caused by mannitol application.ConclusionsWe conclude that NtCIPK11 promotes plant growth and mitigates damage associated with salt stress by regulating the expression of genes controlling proline accumulation. These results extend our understanding on the function of halophyte-derived CIPK genes and suggest that NtCIPK11 can serve as a candidate gene for improving the salt and drought tolerance of glycophytes through genetic engineering.