文献类型：期刊文献

英文题名：PagKNAT2/6b promotes shoot branching by attenuating auxin-strigolactone signalling in poplar

作者：Zhao, Yan-Qiu[1,2] Song, Xue-Qin[3] Guo, Wei[4] Jiang, Cheng[1] Zhang, Jin[1] Lu, Meng-Zhu[1,3]

第一作者：Zhao, Yan-Qiu

通信作者：Lu, MZ[1]

机构：[1]Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Key Lab Modern Silvicultural Technol Zhejiang Pro, Hangzhou 311300, Zhejiang, Peoples R China;[2]Ludong Univ, Inst Agr & Forestry, Engn Res, Yantai, Shandong, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing, Peoples R China;[4]Taishan Acad Forestry Sci, Tainan, Taiwan

年份：2024

卷号：47

期号：10

起止页码：3920-3935

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-85195501101);WOS:【SCI-EXPANDED(收录号:WOS:001241635600001)】；

基金：This study was supported by the National Key Research and Development Program of China (2021YFD2200205), the National Science Foundation of China (32101482 and 32101478), the Youth Innovation Technology Project of Higher School in Shandong Province (2022KJ120), the Zhejiang A&F University Research and Development Fund Talent Startup Project (2018FR013 and 2021LFR013) and the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties (2021C02070-1).

语种：英文

外文关键词：branch development; IAA-SL; PagYUC6a; plant architecture; Populus

摘要：Shoot branching from axillary bud (AB) directly determines plant architecture. However, the mechanism through which AB remains dormant or emerges to form branches as plants grow remains largely unknown. Here, the auxin-strigolactone (IAA-SL) pathway was first shown to regulate shoot branching in poplar, and we found that PagKNAT2/6b could modulate this pathway. PagKNAT2/6b was expressed mainly in the shoot apical meristem and AB and was induced by shoot apex damage. PagKNAT2/6b overexpressing poplar plants (PagKNAT2/6b OE) exhibited multiple branches that mimicked the branching phenotype of nontransgenic plants after decapitation treatment, while compared with nontransgenic controls, PagKNAT2/6b antisense transgenic poplar and Pagknat2/6b mutant lines exhibited a significantly decreased number of branches after shoot apex damage treatment. In addition, we found that PagKNAT2/6b directly inhibits the expression of the key IAA synthesis gene PagYUC6a, which is specifically expressed in the shoot apex. Moreover, overexpression of PagYUC6a in the PagKNAT2/6b OE background reduced the number of branches after shoot apex damage treatment. Overall, we conclude that PagKNAT2/6b responds to shoot apical injury and regulates shoot branching through the IAA-SL pathway. These findings may provide a theoretical basis and candidate genes for genetic engineering to create new forest tree species with different crown types.