文献类型：期刊文献

英文题名：Comparative Transcriptome and Pigment Analyses Reveal Changes in Gene Expression Associated with Flavonol Metabolism in Yellow Camellia

作者：Liu, Weixin[1] Yu, Suhang[1] Feng, Yi[1] Mo, Runhong[1] Wang, Kaineng[2] Fan, Menglong[1] Fan, Zhengqi[1] Yin, Hengfu[1] Li, Jiyuan[1] Li, Xinlei[1]

第一作者：刘伟鑫

通信作者：Li, XL[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Construct Technician Coll, Hangzhou 311400, Peoples R China

年份：2022

卷号：13

期号：7

外文期刊名：FORESTS

收录：;EI(收录号：20223612694235);Scopus(收录号：2-s2.0-85137151549);WOS:【SCI-EXPANDED(收录号:WOS:000832111000001)】；

基金：This work was funded by National Key R&D Program of China (2020YFD1000500), Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02071-2) and Fundamental Research Funds of CAF (CAFYBB2021QD001-1).

语种：英文

外文关键词：Camellia nitidssima; hybrids; flower color; transcriptome; flavonol; ethylene

摘要：The accumulation of various pigments leads to the formation of different flower colors in plants. However, the regulation mechanism of yellow flower formation and flower color differences between Camellia nitidssima C.W.Chi (CN) and its hybrids C. 'Zhenghuangqi' (ZHQ), C. 'Huangxuanlv' (HXL), and C. 'Xinshiji' (XSJ), remains largely unknown. Here, we showed that the content of two flavonols, quercetin-7-O-glucoside (Qu7G) and quercetin-3-O-glucoside (Qu3G), was positively correlated with the yellow degree of petals in CN and its three hybrids. Additionally, we performed a comparative transcriptomic analysis of petals of the four yellow camellia plants, which revealed 322 common upregulated and 866 common downregulated DEGs (differentially expressed genes) in the CN vs. ZHQ, CN vs. HXL, and CN vs. XSJ comparison groups. Their regulatory pathway analysis showed that flavonol biosynthesis genes (FLSs and GTs) and transcriptional regulatory genes MYBs were all expressed higher in CN than its three hybrids, which corresponded to differences in the flavonol content among the four yellow camellias. Further, two ethylene synthesis genes (ACSs, ACO) and three ethylene signaling genes (EIN2s, EIN3, ERFs) were all upregulated in the yellow petals of CN. In conclusion, the expression of flavonol-related genes and flavonols (Qu7G and Qu3G) accumulation could play a key role in the formation of yellow flowers in camellia, and the ethylene pathway might be involved in the regulation of yellow flower formation of camellias. This work describes the possible regulatory pathway of yellow camellia, thereby laying a foundation for future verification of genes linked to flower coloring and the breeding of yellow camellia.