文献类型：期刊文献

英文题名：TWENTY MICROSATELLITE MARKERS FOR THE ENDANGERED VATICA MANGACHAPOI (DIPTEROCARPACEAE)

作者：Guo, Jun-Jie[1] Shang, Shuai-Bin[2] Wang, Chun-Sheng[1] Zhao, Zhi-Gang[1] Zeng, Jie[1]

第一作者：郭俊杰

通信作者：Zeng, J[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]State Forestry Adm, Northwest Inst Forest Inventory Planning & Design, Xian 710048, Shaanxi, Peoples R China

年份：2017

卷号：5

期号：2

外文期刊名：APPLICATIONS IN PLANT SCIENCES

收录：;Scopus(收录号：2-s2.0-85011867550);WOS:【SCI-EXPANDED(收录号:WOS:000395675500006)】；

语种：英文

外文关键词：conservation genetics; cross-species transferability; Dipterocarpaceae; microsatellite markers; next-generation sequencing; Vatica mangachapoi

摘要：center dot Premise of the study: Microsatellite markers were developed for Vatica mangachapoi (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China. center dot Methods and Results: Twenty microsatellite markers, including 12 polymorphic markers, were identified from V. mangachapoi using high- throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species V. guangxiensis. center dot Conclusions: These markers may be used to investigate the genetic diversity and gene flow of V. mangachapoi and V. guangxiensis.