文献类型：期刊文献

英文题名：Galla chinensis residue-derived carbon dots for sensitive detection of doxorubicin hydrochloride and cell imaging

作者：Tang, Baoshan[1,3] Xiong, Fen[4] Liu, Lanxiang[1,2] Xu, Juan[1,2] Ma, Jinju[1,2] Zhang, Hong[1]

第一作者：Tang, Baoshan

机构：[1] Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, 650223, China; [2] Research Center of Engineering and Technology of Characteristic Forest Resources, National Forestry and Grassland Administration. Key Laboratory of Breeding and Utilization of Resource Insects, National Forestry and Grassland Administration, Kunming, 650223, China; [3] Graduate school, Nanjing Forestry University, Nanjing, 210037, China; [4] College of Forestry, Southwest Forestry University, Kunming, 650224, China

年份：2024

卷号：414

外文期刊名：Journal of Molecular Liquids

收录：EI(收录号：20243917088086);Scopus(收录号：2-s2.0-85204470104)

语种：英文

外文关键词：Fluorescence imaging - Fluorescence quenching - Fluorescence spectroscopy

摘要：As an antibiotic, the imbalance of the concentration of doxycycline hydrochloride (DOX) can cause many harms, so real-time detection of the DOX content is crucial. In this study, Galla chinensis residue (GCR) was used as a carbon source, m-phenylenediamine was used as an N-doping agent, and blue-green fluorescent carbon dots (N-GCRCDs) were prepared by a simple hydrothermal synthesis method, with a high quantum yield of 27.31 %. Under optimized conditions, a specific fluorescent probe was established to detect DOX. The addition of DOX resulted in a significant decrease in fluorescence intensity, with a detection range of 5.7–114.9 μM and the detection limit was 1.38 μM. Finally, the feasibility of this method was validated in human urine and the experimental results exhibited a DOX recovery rate of 97.45–104.41 % and an RSD of 0.91–2.64 %. The quenching mechanism was mainly attributed to the inner filter effect and static quenching. Furthermore, the potential application of N-GCRCDs in cells was studied and biocompatibility was evaluated using HepG2 cells, which showed low toxicity at concentrations of 0–50 μg/mL and successful application for HepG2 cell imaging. This study provides reference for resource utilization of GCR and quantitative detection of antibiotics. ? 2024 Elsevier B.V.