文献类型：期刊文献

英文题名：Peclh2 Gene Positively Regulate Salt Tolerance in Transgenic Populus Alba × Populus Glandulosa

作者：Ge, Xiaolan[1,2] Du, Jiujun[1] Zhang, Lei[1] Wen, Shuangshuang[3] He, Hui[4] Qu, Guanzheng[2] Hu, Jianjun[1,5]

第一作者：Ge, Xiaolan

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Road, Haidian, Beijing, 100091, China; [2] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Hexing Road, Heilongjiang, Harbin, 150040, China; [3] Institute of Subtropical Crops of Zhejiang Province, 334 Xueshan Road, Zhejiang, Wenzhou, 325005, China; [4] College of Forestry, Xinyang Agriculture and Forestry University, Henan, Xinyang, 464000, China; [5] Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220344453)

语种：英文

外文关键词：Cloning - Physiology - Plants (botany) - RNA

摘要：Salt is an important environmental stress factor, which seriously affects the growth, development and distribution of plants. Chlorophyllase plays an important role in stress response. Nevertheless, little is known about the physiological and molecular mechanism of Chlorophyll (Chlase, CLH ) genes to plants. We cloned PeCLH2 from Populus euphratica , and found that PeCLH2 was differentially expressed in different tissues, especially in the root of P. euphratica . PeCLH2 protein localized to the nucleus. To further study the role of PeCLH2 in salt tolerance, PeCLH2 overexpression and RNA interference transgenic lines were established in Populus alba × Populus glandulosa , and used for salt stress treatment and physiologic indexes studies. Overexpressing lines significantly improved tolerance to salt treatment and reduced reactive oxygen species production. RNA interference lines showed the opposite. Transcriptome analysis was performed on leaves of control and transgenic lines under normal growth conditions and salt stress to predict genes regulated during salt stress. This provides a basis for elucidating the molecular regulation mechanism of PeCLH2 in response to salt stress and improving the tolerance of poplar under salt stress. ? 2022, The Authors. All rights reserved.