文献类型：期刊文献

中文题名：落叶松体细胞胚萌发能力研究

英文题名：Germination Ability of Somatic Embryos in Larix leptolepis

作者：李哲馨[1] 李万峰[1] 韩素英[2] 齐力旺[1]

第一作者：李哲馨

机构：[1]中国林业科学研究院林业研究所细胞生物学实验室;[2]中国林业科学研究院森林生态环境与保护研究所

年份：2017

卷号：30

期号：6

起止页码：999-1003

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金："十二五"国家高技术研究发展计划(863)重点项目"白桦;落叶松转基因育种技术研究"(2013AA102704);国家自然科学基金重点项目"落叶松干细胞发育关键MicroRNAs功能分析及其TALE定向突变体创制"(31330017)

语种：中文

中文关键词：落叶松;体细胞胚再生体系;萌发率;IAA

外文关键词：Larix leptolepis ; somatic embryogenesis system ; germination rate ; IAA

分类号：S791.223

摘要：[目的]研究落叶松体细胞胚萌发能力,为落叶松在应用方面规模化繁殖提供理论依据。[方法]以落叶松体胚性细胞系S287的成熟体细胞胚为材料,区分了落叶松体细胞胚的不同子叶形态;进行树脂切片及显微观察后,对其萌发能力进行了统计;并利用外源施用激素的方法研究了影响落叶松体胚再生成苗的重要因子。[结果]成熟的落叶松体细胞胚主要以四种形态存在,分别是正常子叶胚(NC≥4),裂生子叶胚(2[Objective] Germination ability of somatic embryos in Larix leptolepis was investigated to provide theoretical basis for large-scale breeding. [Method] The study was conducted by using mature somatic embryos （SEs） from embryonic cell line S287 of L. leptolepis as the material. Four cotyledon shapes were distinguished and analyzed with resin sections method and microscopic observation. The rooting ability of the four types of SEs was calculated and the important factors that affect seedlings regeneration were identified with exogenous application of hormones. [Result] Four types of mature SEs in L. leptolepis were found in the investigation, i.e. the normal one with cotyledon number （NC） ≥ 4, the poorly separated-cotyledon one with 2〈 NC〈 4, the cup shape one with NC=1 and the deformed one. Microscopic observation revealed that the first three SEs could develop complete SAM and RAM, with germination rates of 17.2%, 12.4% and 5.7% respectively. However, the abnormal embryos did not have full SAM and RAM, and could not take root and soon died in germination medium. Exogenous application with GA3 and IAA of different concentration showed that 2 mg·L^-1 IAA obviously promote the germination ability with 56% germination rate of SEs in L. leptolepis and later maintain a higher survival rate.[Conclusion] IAA plays a vital role in modulating germination ability of somatic embryos in L. leptolepis.