文献类型：期刊文献

中文题名：京2杨组培条件的优化及再生体系建立

英文题名：Optimization of Tissue Culture Conditions and Establishment of Regeneration System for Populus×euramericana cl.'J2'

作者：张蕾[1] 苏晓华[2] 张冰玉[2] 黄秦军[2] 张香华[2] 李义良[2]

第一作者：张蕾

机构：[1]沈阳农业大学林学院;[2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2007

卷号：20

期号：6

起止页码：787-793

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家科技支撑计划课题"高产优质杨树;桉树等速生材树种新品种选育"(编号2006BAD01A15)

语种：中文

中文关键词：京2杨;组织培养;再生体系

外文关键词：P. × euramericana cl.‘ J2＇ ; tissue culture ; regeneration system

分类号：S722.3

摘要：以京2杨为研究对象,确定了影响其组培苗生长的关键因子及组培苗生长的最佳条件,并为其建立了稳定高效的再生体系。试验结果表明:京2杨组织培养的最适基本培养基为MS培养基,继代培养基和不定芽最佳生根培养基均为1/2MS+NAA 0.08 mg.L-1+IBA 0.08 mg.L-1;培养基中加入活性碳不利于京2杨生长;光周期对京2杨的生长影响较小,但光照强度和继代培养基pH值对其生长影响显著,最适光照强度为2 500 lx,最适pH值为6.5;无菌苗叶片最佳不定芽诱导培养基为MS+BA 1.0 mg.L-1+NAA 0.1 mg.L-1,叶柄最佳不定芽诱导培养基为MS+BA 1.0 mg.L-1+NAA 0.2 mg.L-1。
The key factors affecting the growth of Populus × euramericana el. ‘J2＇ in vhro and the optimal tissue culture conditions for it were ascertained. The high frequency regeneration system for P.×euramericana cl. ‘J2＇ was also established in this study. The optimum basic culture medium was MS;the optimal subculture medium and root inducement culture medium for shoot were 1/2MS ＋ NAA 0.08 mg· L^-1 ＋ IBA 0.08·mg L^-1 ; active carbon disadvantaged the growth of P.× euramericana cl. ‘ J2＇ ; photoperiod had little effect on P. × euramericana cl. ‘ J2＇ , while light intensity and the pH value of subculture medium had great effect on it, the optimal light intensity was 2 500 Ix and the optimal pH value was 6.5 ; the optimal shoot inducement culture medium for leaf-explant was MS ＋ BA1.0 mg· L ^-1 ＋ NAA0.1 mg · L^-1 and for leafstalk-explant was MS ＋ BA 1.0 mg · L^-1 ＋ NAA 0.2 mg ·L^-1.