文献类型：期刊文献

英文题名：Identification of key gene networks related to the freezing resistance of apricot kernel pistils by integrating hormone phenotypes and transcriptome profiles

作者：Liu, Xiaojuan[1] Xu, Huihui[1] Yu, Dan[1] Bi, Quanxin[1] Yu, Haiyan[1] Wang, Libing[1]

第一作者：刘肖娟

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2022

卷号：22

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85141886520);WOS:【SCI-EXPANDED(收录号:WOS:000884895300002)】；

基金：This work was financially supported by the Central Public-Interest Scientific Institution Basal Research Fund (CAFYBB2019SY005), the National Key Research and Development Program of China (2018YFD1000606-33) and the "Youth Top Talent project" of the "Ten Thousand Talents Program" of the state.

语种：英文

外文关键词：Apricot kernel; Freezing stress; Hormone; Transcriptome; Auxin; Gene network

摘要：Background Apricot kernel, a woody oil tree species, is known for the high oil content of its almond that can be used as an ideal feedstock for biodiesel production. However, apricot kernel is vulnerable to spring frost, resulting in reduced or even no yield. There are no effective countermeasures in production, and the molecular mechanisms underlying freezing resistance are not well understood. Results We used transcriptome and hormone profiles to investigate differentially responsive hormones and their associated co-expression patterns of gene networks in the pistils of two apricot kernel cultivars with different cold resistances under freezing stress. The levels of auxin (IAA and ICA), cytokinin (IP and tZ), salicylic acid (SA) and jasmonic acid (JA and ILE-JA) were regulated differently, especially IAA between two cultivars, and external application of an IAA inhibitor and SA increased the spring frost resistance of the pistils of apricot kernels. We identified one gene network containing 65 hub genes highly correlated with IAA. Among these genes, three genes in auxin signaling pathway and three genes in brassinosteroid biosynthesis were identified. Moreover, some hub genes in this network showed a strong correlation such as protein kinases (PKs)-hormone related genes (HRGs), HRGs-HRGs and PKs-Ca2+ related genes. Conclusions Ca2+, brassinosteroid and some regulators (such as PKs) may be involved in an auxin-mediated freezing response of apricot kernels. These findings add to our knowledge of the freezing response of apricot kernels and may provide new ideas for frost prevention measures and high cold-resistant apricot breeding.