文献类型：期刊文献

英文题名：Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

作者：Ma, H. B.[1,2] Lu, Q.[1] Liang, J.[1] Zhang, X. Y.[1]

第一作者：马海宾

通信作者：Zhang, XY[1]|[a000532db0686e0c7a9f9]张星耀;

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou, Guangdong, Peoples R China

年份：2011

卷号：10

期号：3

起止页码：1931-1941

外文期刊名：GENETICS AND MOLECULAR RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000295823500063)】；

基金：Research supported by the Common Weal Research Program for Forestry (#200904061) and the International S&T Cooperation Program of China (#2007DFB30270).

语种：英文

外文关键词：Bursaphelenchus xylophilus; Endo-beta-1,4-glucanase; RNA interference

摘要：Cellulases are pathogenic substances suspected to be responsible for the development of the early symptoms of nematode disease. The pine wood nematode, Bursaphelenchus xylophilus (Parasitaphelenchidae), is the causal agent of pine wilt disease, which kills millions of pine trees. We used RNA interference (RNAi), a reverse genetic tool, to analyze the function of the endo-beta-1,4-glucanase gene of B. xylophilus, which causes the most serious forest tree disease in China and the rest of eastern Asia. Silencing of this gene was detected through real-time PCR and cellulase activity assays after soaking for 24 h in dsRNA. The cellulase gene silencing effects differed among various siRNAs. The propagation and dispersal ability of these nematodes decreased when the endo-beta-1,4-glucanase gene was silenced. It is important to select an effective siRNA before performing an RNAi test.