文献类型：期刊文献

英文题名：Pseudomonas quercus sp. nov, associated with leaf spot disease of Quercus mongolica

作者：Li, Yong[1] Wang, Shengkun[2] Bian, Danran[1] Sun, Shang[1] Ma, Tengfei[1]

通信作者：Li, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol, Key Lab Forest Protect, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

年份：2021

卷号：71

期号：5

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85105891795);WOS:【SCI-EXPANDED(收录号:WOS:000663082600002)】；

基金：The authors wish to acknowledge the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018ZB001) , and the National Infrastructure of Microbial Resources (NIMR-2019-7) from the Ministry of Science and Technology of the People's Republic of China.

语种：英文

外文关键词：multilocus sequence analysis; average nucleotide identity; Pseudomonas; Quercus mongolica

摘要：Two Gram-negative, aerobic, motile bacteria strains were isolated from leaf spot disease of Quercus mongolica. Strain hsmgli-8(T) has 99.86 % 16S rRNA gene sequence similarity to LY10J, and the highest 16S rRNA gene sequence similarity to Pseudomonas cerasi 58(T) (97.2 %), then Pseudomonas ficuserectae JCM 2400(T) (97.18 %), Pseudomonas meliae CFBP 3225(T), Pseudomonas tremae CFBP 6111(T) and Pseudomonas congelans DSM 14939(T) (all 97.12 %), and less than 97.1 % similarity to other recognized species. In phylogenetic trees based on 16S rRNA gene and multilocus sequence data, the two novel strains form a separate branch, indicating that they do not belong to any Pseudomonas group and subgroup, and should belong to a novel species within the genus Pseudomonas. This assertion is also supported by the results of genome average nucleotide identity analysis. The major fatty acids are C-16:0, C-18:1 omega 7c and/or C-18:1 omega 6c, C-16:1 omega 7c and/or C-16:1 omega 6c. Polar lipids include phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, aminolipid and seven uncharacterized phospholipids. The predominant respiratory quinone is Q-9. The DNA G+C content is 59.45-59.50 mol%. Based on these data, we propose that the two novel strains should be assigned as a novel species within the genus Pseudomonas. We propose that the novel strains be named Pseudomonas quercus sp. nov. The type strain is hsmgli-8(T) (=CFCC 15739(T)=LMG 31544(T)).