文献类型：期刊文献

英文题名：An improved draft genome sequence of hybridPopulus alba x Populus glandulosa

作者：Huang, Xiong[1,2] Chen, Song[3] Peng, Xiaopeng[2] Bae, Eun-Kyung[4] Dai, Xinren[1] Liu, Guiming[5] Qu, Guanzheng[3] Ko, Jae-Heung[6] Lee, Hyoshin[4] Chen, Su[3] Li, Quanzi[1,2] Lu, Mengzhu[2]

第一作者：Huang, Xiong

通信作者：Li, QZ[1];Li, QZ[2];Lu, MZ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[4]Natl Inst Forest Sci, Div Forest Biotechnol, Suwon 441847, South Korea;[5]Beijing Acad Agr & Forestry Sci, Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China;[6]Kyung Hee Univ, Dept Plant & Environm New Resources, Yongin 446701, South Korea

年份：2021

卷号：32

期号：4

起止页码：1663-1672

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000579719200001)】；

基金：This work was supported by grants CAFYBB2017ZY001 and TGB2016001 from Fundamental Research Funds of the Chinese Academy of Forestry.

语种：英文

外文关键词：Genome assembly; Gene annotation; Hybrid poplar; Populus alba x p; glandulosacl; 84 K

摘要：Populus alba x P. glandulosaclone 84 K, derived from South Korea, is widely cultivated in China and used as a model in the molecular research of woody plants because of high gene transformation efficiency. Here, we combined 63-fold coverage Illumina short reads and 126-fold coverage PacBio long reads to assemble the genome. Due to the high heterozygosity level at 2.1% estimated byk-mer analysis, we exploited TrioCanu for genome assembly. The PacBio clean subreads ofP. alba x P. glandulosawere separated into two parts according to the similarities, compared with the parental genomes ofP. albaandP. glandulosa. The two parts of the subreads were assembled to two sets of subgenomes comprising subgenome A (405.31 Mb, fromP. alba) and subgenome G (376.05 Mb, fromP. glandulosa) with the contig N50 size of 5.43 Mb and 2.15 Mb, respectively. A high-qualityP. alba x P. glandulosagenome assembly was obtained. The genome size was 781.36 Mb with the contig N50 size of 3.66 Mb and the longest contig was 19.47 Mb. In addition, a total of 176.95 Mb (43.7%), 152.37 Mb (40.5%) of repetitive elements were identified and a total of 38,701 and 38,449 protein-coding genes were predicted in subgenomes A and G, respectively. For functional annotation, 96.98% of subgenome A and 96.96% of subgenome G genes were annotated with public databases. This de novo assembled genome will facilitate systematic and comprehensive study, such as multi-omics analysis, in the model treeP. alba x P. glandulosa.