文献类型：期刊文献

英文题名：Genome Comparison Reveals Mutation Hotspots in the Chloroplast Genome and Phylogenetic Relationships of Ormosia Species

作者：Liu, Hongshan[1,2] Su, Zhihai[2] Yu, Shuiqing[2] Liu, Jialin[2] Yin, Xiaojuan[2] Zhang, Guowei[2] Liu, Wei[2] Li, Bin[1]

第一作者：Liu, Hongshan

通信作者：Li, B[1]

机构：[1]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Key Lab Tree Breeding & Forest Genet, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Adm Bur Hongyashan State Owned Forest Farm Hebei, Yixian 074200, Peoples R China

年份：2019

卷号：2019

外文期刊名：BIOMED RESEARCH INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000484707000001)】；

基金：LThis research was funded by the "cooperation project between CAF and the Province (2019sy03)." The authors would like to acknowledge and thank Dr. Yanlei Liu and Dr. Chao Xu at IBCAS (Institute of Botany, Chinese Academy of Sciences) for technical support with the Illumina sequencing.

语种：英文

摘要：The papilionoid legume genus Ormosia comprises approximately 130 species, which are distributed mostly in the Neotropics, with some species in eastern Asia and northeastern Australia. The taxonomy and evolutionary history remain unclear due to the lack of a robust species-level phylogeny. Chloroplast genomes can provide important information for phylogenetic and population genetic studies. In this study, we determined the complete chloroplast genome sequences of five Ormosia species by Illumina sequencing. The Ormosia chloroplast genomes displayed the typical quadripartite structure of angiosperms, which consisted of a pair of inverted regions separated by a large single-copy region and a small single-copy region. The location and distribution of repeat sequences and microsatellites were determined. Comparative analyses highlighted a wide spectrum of variation, with trnK-rbcL, atpE-trnS-rps4, trnC-petN, trnS-psbZ-trnG, trnP-psaJ-rpl33, and clpP intron being the most variable regions. Phylogenetic analysis revealed that Ormosia is in the Papilionoideae clade and is sister to the Lupinus clade. Overall, this study, which provides Ormosia chloroplast genomic resources and a comparative analysis of Ormosia chloroplast genomes, will be beneficial for the evolutionary study and phylogenetic reconstruction of the genus Ormosia and molecular barcoding in population genetics and will provide insight into the chloroplast genome evolution of legumes.