文献类型：期刊文献

中文题名：不同树龄侧柏古树扦插过程中的抑制物研究

英文题名：Inhibitors during cutting process of ancient Platycladus orientalis trees of different ages

作者：魏黔春[1] 刘建锋[1] 郭伟[2] 王鹤智[3] 徐健楠[3] 晁碧霄[3] 常二梅[1]

第一作者：魏黔春

机构：[1]林木遗传育种全国重点实验室,国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]泰安市泰山林业科学研究院,山东泰安271000;[3]国家林业和草原局林草调查规划院,北京100714

年份：2024

卷号：46

期号：11

起止页码：83-91

中文期刊名：北京林业大学学报

外文期刊名：Journal of Beijing Forestry University

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：“十四五”国家重点研发计划课题(2022YFD2200103);国家自然科学基金面上项目(32471905);中央级公益性科研院所基本科研业务费专项重点项目(CAFYBB2022ZA001)。

语种：中文

中文关键词：侧柏;古树;扦插;抑制物;生根

外文关键词：Platycladus orientalis;ancient tree;cuttings;inhibitors;rooting

分类号：S791.38

摘要：【目的】探究不同树龄侧柏古树在扦插过程中的内源抑制物(如酚酸和类黄酮)对生根的影响,为提高侧柏古树扦插生根率提供理论依据。【方法】研究3月份和6月份不同树龄(5、100、300和700年生)侧柏的插穗浸提液对白菜籽发芽的影响,并且测定插穗酚酸、类黄酮物质的含量。比较6月份不同树龄侧柏插穗在扦插生根过程中的酚酸、类黄酮物质对生根过程的影响。筛选去除100年生侧柏插穗抑制物的方法。【结果】(1)侧柏古树(100、300和700年生)的生根率和生根数量显著低于幼年侧柏(5年生)(P<0.05)。(2)3月份100、300和700年生侧柏插穗的浸提液对白菜籽发芽的抑制作用显著高于6月份(P<0.05)。随着树龄的增长,插穗的浸提液对白菜籽发芽的抑制作用增强。(3)6月份侧柏插穗的酚酸和类黄酮含量普遍较低。在3月份和6月份,芦丁、水杨酸、没食子酸、香豆酸、阿魏酸的含量随侧柏树龄的增长总体上呈增加趋势。在6月份,5、100、300、700年生侧柏的插穗酚酸和类黄酮含量随生根阶段呈增加趋势。(4)0.1%硝酸银溶液处理侧柏古树插穗能促进不定根形成,0.05%高锰酸钾溶液能促进根系发育。【结论】随着侧柏树龄的增长,插穗中酚酸和类黄酮含量的增加对不定根形成产生了抑制作用。然而,通过使用0.1%硝酸银或0.05%高锰酸钾处理,可有效缓解这一抑制,提高侧柏扦插生根率。本文为提高难生根林木扦插生根率提供了理论依据和技术支撑。
[Objective]This paper explores the effects of endogenous inhibitors(such as phenolic acids and flavonoids)produced by ancient Platycladus orientalis trees of different ages on rooting during the cutting process,so as to provide a theoretical basis for improving the rooting rate of ancient P.orientalis cuttings.[Method]The cuttings from 5-,100-,300-,and 700-year-old P.orientalis were used as experimental materials for cutting propagation.The effects of extracts from the cuttings of P.orientalis of different ages(5-,100-,300-,and 700-year-old)in March and June on the germination of cabbage seeds were studied,and the contents of phenolic acids and flavonoids in the cuttings were measured to compare the effects of phenolic acids and flavonoids produced during the rooting process of cuttings of P.orientalis trees of different ages from June on the rooting process.Screening different removal methods was to identify the optimal approach for eliminating inhibitors from 100-year-old P.orientalis cuttings.[Result](1)The rooting rates and root numbers of ancient P.orientalis trees(100-,300-,and 700-year-old)were significantly lower than those of young P.orientalis trees(5-year-old)(P<0.05).(2)The inhibitory effects of extracts from 100-,300-,and 700-year-old P.orientalis cuttings on cabbage seed germination in March were significantly higher than that in June(P<0.05).As the ages of trees increased,the inhibitory effects of extracts of cuttings on inhibiting the germination of cabbage seeds increased.(3)The contents of phenolic acids and flavonoids in P.orientalis cuttings from June were generally low.In the experimental materials from March and June,the contents of rutin,salicylic acid,gallic acid,coumaric acid,and ferulic acid generally showed an increasing trend with the increase of age of P.orientalis.In the experimental materials from June,the phenolic acid and flavonoid contents of 5-,100-,300-,and 700-year-old P.orientalis cuttings showed an increasing trend with the development of rooting process.(4)Treating cuttings of ancient P.orientalis with 0.1%silver nitrate solution can promote the formation of adventitious roots,and treating them with 0.05%potassium permanganate solution can promote root development.[Conclusion]As the age of P.orientalis grows,the phenolic acid and flavonoid contents of cuttings increase,which have inhibitory effects on the formation of adventitious roots.However,treatment of P.orientalis cuttings with 0.1%silver nitrate or 0.05%potassium permanganate can effectively alleviate this inhibitory effect and improve the rooting rates of P.orientalis cuttings.The study provides theoretical basis and technical support for improving rooting rates.