文献类型：期刊文献

英文题名：Pathogenesis-related protein PR10 from Salix matsudana Koidz exhibits resistance to salt stress in transgenic Arabidopsis thaliana

作者：Han, Xiaojiao[1,2] He, Xuelian[1,2] Qiu, Wenmin[1,2] Lu, Zhuchou[3] Zhang, Yunxing[1,2] Chen, Shuangshuang[1,2] Liu, Mingying[1,2] Qiao, Guirong[1,2] Zhuo, Renying[1,2]

第一作者：韩小娇

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Hubei, Peoples R China

年份：2017

卷号：141

起止页码：74-82

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000410017100008)】；

基金：This work was supported by National Natural Science Foundation of China [No. 31300508 and 31370600], Agricultural Projects of Public Scientific and Technology Research Zhejiang Province [2016C32G3030016], and a National Nonprofit Institute Research Grant of CAF [No. CAFYBB2014QB014 and CAFYBB2017ZY007].

语种：英文

外文关键词：Pathogenesis-related protein 10; Salix matsudana; Salt stress; Overexpression

摘要：Pathogenesis-related (PR) proteins are involved in plant defense and have multiple functional adaptations that assist in resisting various pathogens and tolerating environmental stress. Salix matsudana Koidz, a deciduous, rapidly growing willow species, can tolerate a range of adverse conditions. Comparative proteomic analysis previously revealed that the S. matsudana PR protein SmPR10 was abundant and up-regulated with 100 mM NaCl treatment. In this study, the SmPR10 gene from S. matsudana was cloned and characterized to determine its role in salt tolerance. The amino acid sequence of SmPR10 showed 98% and 93% sequence homology with PR proteins from S. purpurea and Populus trichocarpa, respectively. SmPR10 was localized in the cytoplasm of Arabidopsis protoplasts. SmPR10 transcript and protein levels were high in roots, and its expression was up regulated in roots treated with 100 mM NaCl. SmPR10 was detected specifically in phloem fiber cells and root xylem by immunolocalization analysis. Moreover, heterogeneous overexpression of SmPR10 enhanced the salt tolerance of transgenic Arabidopsis plants as shown by analysis of root length, root number, and Na+ flux, as well as physiological parameters such as chlorophyll content, MDA content, electrical conductivity, and SOD and POD enzyme activity levels. Our results reveal that SmPR10 plays an important role in salt tolerance and could serve as an important candidate gene to improve salt tolerance in woody species through genetic engineering.