文献类型：期刊文献

中文题名：Loss of the accessory chromosome converts a pathogenic tree-root fungus into a mutualistic endophyte

作者：Huanshen Wei[1,2] Zhenhui Zhong[3] Zhongfeng Li[1,2] Yuwei Zhang[1,2] Eva H.Stukenbrock[4,5] Boping Tang[6] Ningning Yang[1,2] Riccardo Baroncelli[7] Long Peng[1,2] Zhuo Liu[1,2] Xinghua He[1,2] Yuzhan Yang[1,2] Zhilin Yuan[1,2]

第一作者：Huanshen Wei

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China;[2]Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;[3]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;[4]Environmental Genomics,Christian-Albrechts University,24118 Kiel,Germany;[5]Max Planck Fellow Group Environmental Genomics,Max Planck Institute for Evolutionary Biology,24306 Plon,Germany;[6]Jiangsu Key Laboratory for Bioresources of Saline Soils,School of Wetlands,Yancheng Teachers University,Yancheng 224002,China;[7]Department of Agricultural and Food Sciences(DISTAL),University of Bologna,40127 Bologna,Italy

年份：2024

卷号：5

期号：1

起止页码：164-180

中文期刊名：Plant Communications

外文期刊名：植物通讯（英文）

收录：Scopus;CSCD:【CSCD2023_2024】；PubMed;

基金：supported by the National Key Research and Development Program of China (2022YFD2201900);the National Natural Science Foundation of China (no.31722014).

语种：英文

中文关键词：dark septate endophytes;effectors;lifestyle transition;root–fungus symbioses

分类号：Q94

摘要：Some fungal accessory chromosomes(ACs)may contribute to virulence in plants.However,the mecha-nisms by which ACs determine specific traits associated with lifestyle transitions along a symbiotic contin-uum are not clear.Here we delineated the genetic divergence in two sympatric but considerably variable isolates(16B and 16W)of the poplar-associated fungus Stagonosporopsis rhizophilae.We identified a-0.6-Mb horizontally acquired AC in 16W that resulted in a mildly parasitic lifestyle in plants.Complete deletion of the AC(D16W)significantly altered the fungal phenotype.Specifically,D16W was morphologically more similar to 16B,showed enhanced melanization,and established beneficial interactions with poplar plants,thereby acting as a dark septate endophyte.RNA sequencing(RNA-seq)analysis showed that AC loss induced the upregulation of genes related to root colonization and biosynthesis of indole acetic acid and melanin.We observed that the AC maintained a more open status of chromatin across the genome,indicating an impressive remodeling of cis-regulatory elements upon AC loss,which potentially enhanced symbiotic effectiveness.We demonstrated that the symbiotic capacities were non-host-specific through comparable experiments on Triticum–and Arabidopsis–fungus associations.Furthermore,the three isolates generated symbiotic interactions with a nonvascular liverwort.In summary,our study suggests that the AC is a suppressor of symbiosis and provides insights into the underlying mechanisms of mutualism with vascular plants in the absence of traits encoded by the AC.We speculate that AC-situ-ated effectors and other potential secreted molecules may have evolved to specifically target vascular plants and promote mild virulence.