文献类型：期刊文献

英文题名：Chloroplast genome-based genetic variation and genetic diversity of Ulmus pumila (Ulmaceae) germplasm

作者：Liu, Kangjia[1] Cui, Hongxin[2] Wu, Lidong[2] Yang, Huimin[2] Cao, Linlin[1] Sun, Jiahui[3] Li, Bin[4] Dong, Wenpan[1] Wang, Yiheng[3]

第一作者：Liu, Kangjia

通信作者：Dong, WP[1];Wang, YH[2]

机构：[1]Beijing Forestry Univ, Sch Ecol & Nat Conservat, Beijing 100083, Peoples R China;[2]Fengning State Owned Forest Farm Management Off, Dengzhazi Forest Farm, Chengde 068350, Peoples R China;[3]China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2025

卷号：233

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20252718721554);Scopus(收录号：2-s2.0-105009612074);WOS:【SCI-EXPANDED(收录号:WOS:001528634300007)】；

基金：This study was supported by the National Key Research and Development Program of China (2023YFC3503801), Fundamental Research Funds for the Centralpublic welfare research institutes (No.ZK2023003), and Hebei Forestry and GrasslandBureau 2024 central financial forest seed cultivation project (No. Ji Lin Cao Fu (202413))

语种：英文

外文关键词：Chloroplast genome; Genetic diversity; Germplasm; Ulmus pumila

摘要：Ulmus pumila is widely distributed in the north temperate zone and has timber and horticultural value. Assessing its genetic diversity could provide valuable insights into the levels and distribution of genetic variation in U. pumila resources, which could aid in the selection of representative and diverse germplasm. In this study, we sequenced the complete chloroplast genome of 51 U. pumila samples from 19 populations and investigated the population structure and genetic diversity of this species. The chloroplast genome of U. pumila exhibits the typical structure of angiosperm chloroplast genomes, characterized by a circular double-stranded structure. We identified 313 single nucleotide variants and 277 indels. Four markers, trnT-psbD, rpl32-trnL, rps16-trnQ, and the ndhA intron, had high variation in the chloroplast genome. Phylogenetic and population structure analyses showed that U. pumila could be divided into two major clusters, showing significant divergence between them. Furthermore, Cluster I was divided into three clades and Cluster II included two clades. The five clades diverged between 4 and 9 million years ago and the effective population size of U. pumila clades has remained relatively stable since the Pliocene, with no significant fluctuations observed. Our study revealed the genetic diversity and differentiation of U. pumila, providing valuable insights into its genetic variation and a basis for the effective conservation of germplasm resources.