文献类型：期刊文献

英文题名：Chromosome-level genome assembly and resequencing of camphor tree (Cinnamomum camphora) provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of Cinnamomum

作者：Wang, Xin-Dong[1,2] Xu, Chun-Yan[3] Zheng, Yong-Jie[1,4] Wu, Yan-Fang[1] Zhang, Yue-Ting[1,2] Zhang, Ting[1] Xiong, Zhen-Yu[2] Yang, Hai-Kuan[1] Li, Jiang[1] Fu, Chao[1] Qiu, Feng-Ying[1] Dai, Xiao-Ying[1] Liu, Xin-Liang[1] He, Xiao-San[1] Zhou, Song-Song[1] Li, Sheng-Xing[1] Fu, Tao[3] Xie, Han[3] Chen, Yan-Ling[3] Zhang, Qian-Qian[3] Wang, Hong-Qi[3] Wang, Yang-Dong[4] Zhou, Cheng[1] Jiang, Xiang-Mei[1]

第一作者：Wang, Xin-Dong

通信作者：Zhou, C[1];Jiang, XM[1];Wang, YD[2]

机构：[1]Jiangxi Acad Forestry, Camphor Engn & Technol Res Ctr Natl Forestry & Gr, Nanchang 330032, Jiangxi, Peoples R China;[2]Jiangxi Acad Forestry, Jiangxi Prov Key Lab Plant Biotechnol, Nanchang 330032, Jiangxi, Peoples R China;[3]BGI Shenzhen, BGI Genom, Shenzhen 518083, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2022

卷号：9

外文期刊名：HORTICULTURE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000910382400037)】；

基金：This work was financially supported by the Natural Science Foundation for Young Scientists of Jiangxi Province, China (20202ACBL215003), the National Natural Science Foundation of China (31460209), and the Found for Fostering Talent of Jiangxi Academe of Forestry (2017512702). We would like to thank Biomarker Technologies Co., Ltd for their assistance in Hi-C sequencing and whole genome resequencing of Cinnamomum, Dr Yi-Cun Chen from the Chinese Academy of Forestry for valuable manuscript suggestions, Dr Yun-Xiao Zhao from the Chinese Academy of Forestry for functional analysis, Dr Zhi-Yong Xie from Huazhong Agricultural University for protein subcellular location, and the functional analysis, Xishuangbanna Tropical Botanical Garden for plant materials.

语种：英文

摘要：Cinnamomum species attract attentions owing to their scents, medicinal properties, and ambiguous relationship in the phylogenetic tree. Here, we report a high-quality genome assembly of Cinnamomum camphora, based on which two whole-genome duplication (WGD) events were detected in the C. camphora genome: one was shared with Magnoliales, and the other was unique to Lauraceae. Phylogenetic analyses illustrated that Lauraceae species formed a compact sister clade to the eudicots. We then performed whole-genome resequencing on 24 Cinnamomum species native to China, and the results showed that the topology of Cinnamomum species was not entirely consistent with morphological classification. The rise and molecular basis of chemodiversity in Cinnamomum were also fascinating issues. In this study, six chemotypes were classified and six main terpenoids were identified as major contributors of chemodiversity in C. camphora by the principal component analysis. Through in vitro assays and subcellular localization analyses, we identified two key terpene synthase (TPS) genes (CcTPS16 and CcTPS54), the products of which were characterized to catalyze the biosynthesis of two uppermost volatiles (i.e. 1,8-cineole and (iso)nerolidol), respectively, and meditate the generation of two chemotypes by transcriptional regulation and compartmentalization. Additionally, the pathway of medium-chain triglyceride (MCT) biosynthesis in Lauraceae was investigated for the first time. Synteny analysis suggested that the divergent synthesis of MCT and long-chain triglyceride (LCT) in Lauraceae kernels was probably controlled by specific medium-chain fatty acyl-ACP thioesterase (FatB), type-B lysophosphatidic acid acyltransferase (type-B LPAAT), and diacylglycerol acyltransferase 2b (DGAT 2b) isoforms during co-evolution with retentions or deletions in the genome.