文献类型：期刊文献

英文题名：Comprehensive analysis of bamboo oligosaccharyltransferases reveals the potential role of PedDGL1-C-mediated PePRX2 glycosylation in shoot lignification

作者：Qiao, Guirong[1,2] Li, Mengyun[2] Ouyang, Longfei[2] He, Shunjie[2] Li, Yanan[2] Xu, Jing[2] Zhuo, Renying[2] Gao, Zhimin[1]

第一作者：乔桂荣;Qiao, Guirong

通信作者：Gao, ZM[1];Zhuo, RY[2]

机构：[1]Int Ctr Bamboo & Rattan, Key Lab Natl Forestry Grassland Adm Bamboo & Rat, Inst Gene Sci & Industrializat Bamboo & Rattan Res, Beijing 100102, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Zhejiang Key Lab Forest Genet & Breeding, Hangzhou 311400, Peoples R China

年份：2025

卷号：236

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20253819192218);Scopus(收录号：2-s2.0-105016238749);WOS:【SCI-EXPANDED(收录号:WOS:001576879600001)】；

基金：Funding This work was supported by the National Key Research Development Program of China (Grant No. 2021YFD2200504) .

语种：英文

外文关键词：Phyllostachys edulis; Oligosaccharyltransferases; N-glycosylation; Shoot growth; Peroxidase

摘要：Protein glycosylation is a crucial post-translational modification in plants, playing vital roles in various physiological processes. Oligosaccharyltransferases (OSTs) are at the core of the N-glycosylation pathway, responsible for transferring oligosaccharide chains to target proteins. Despite the ecological and economic significance of bamboo as a class of fast-growing plants, the genomic organization and functional diversification of OST family remain poorly characterized in bamboo species. Through comprehensive phylogenomic analysis across 12 bamboo genomes, we identified 227 OST members and revealed that gene family expanded primarily through paleopolyploidization events, with functional conservation maintained under purifying selection. Our systematic investigation of 21 Phyllostachys edulis OST members demonstrated tissue-specific expression patterns during shoot fast growth stages, exhibiting spatiotemporal regulation correlated with culm lignification. Subcellular localization confirmed one OST (PedDGL1-C) targeted endoplasmic reticulum, consistent with its canonical role in N-glycosylation. Protein interaction assays (Y2H, LCA, BiFC and Co-IP) established physical association of PedDGL1-C with one lignification-related class III peroxidase PePRX2. Mass spectrometric analysis identified two conserved N-glycosylation sites (N102 and N216) on PePRX2. Glycosite mutation of N102A, N216A, and N102A/ N216A showed reduced molecular weights while maintaining unchanged subcellular localization of PePRX2. Strikingly, N102 mutation not only disrupted PedDGL1-C-PePRX2 interaction but also caused significant attenuation of peroxidase activity, suggesting a mechanistic link between OST-mediated glycosylation and enzyme function. This study provides the first genome-wide characterization of OSTs in bamboo, demonstrating that PedDGL1-C orchestrates shoot growth through glycosylation-dependent regulation of PePRX2. Our findings advance understanding of post-translational regulatory networks underlying rapid bamboo growth and offer new insights into protein glycosylation mechanisms in monocots.