文献类型：期刊文献

中文题名：核桃离体繁殖技术

英文题名：Micropropagation Techniques for Three Walnut Cultivars

作者：吕守芳[1] 闫爱玲[2] 吴燕民[3] 奚声珂[1]

第一作者：吕守芳

机构：[1]中国林科院林业所;[2]北京市农林科学院林果所;[3]中国农科院生物技术中心

年份：2004

卷号：22

期号：1

起止页码：12-14

中文期刊名：经济林研究

外文期刊名：Economic Forest Researches

收录：CSTPCD

基金：国家林业局"核桃引种研究"课题的部分内容 ;北京市青年科技骨干培养基金资助项目"核桃品种离体繁殖技术的研究"。

语种：中文

中文关键词：核桃;离体繁殖技术;生根诱导;品种类型

外文关键词：walnut cultivar; in vitro propagation; rooting inducement

分类号：S664.1

摘要：对核桃JuglansregiaL.辽核1号、辽核4号、元丰3个品种的茎段进行诱导芽分化、生长和生根成苗试验。筛选出茎段离体初代培养基为DKW+BA1.0mg/L;继代培养和壮苗培养基为DKW+BA1.0mg/L+IBA0.01mg/L,诱导生根培养基为1/2DKW+IBA5mg/L,加活性碳1mg/L和间苯三酚1mg/L,生根率达95%以上,重复性稳定。
Three walnut cultivars of 'Liaohe 1', 'Liaohe 4', and 'Yuanfeng' were propagated in vitro. Stem fragments were used for bud differentiation, bud growth, and plantlet rooting. Explantlets were initiated on basal DKW with hormone (BA 1.0 mg/L). The new shoots that had emerged from the primary buds were successively transferred to DKW with hormones (BA 1.0 mg/L and IBA 0.01 mg/L). The rooting medium was 1/2 DKW with hormone (IBA 5 mg/L) plus active carbon at 1.0 mg/L and phloroglucinol at 1.0 mg/L, and the rooting percentage exceeded 95 percent with stable repetitiveness.