文献类型：期刊文献

英文题名：Overexpression of a peroxiredoxin gene from Tamarix hispida, ThPrx1, confers tolerance to oxidative stress in yeast and Arabidopsis

作者：Wang, Liuqiang[1] Li, Zhen[1] Wang, Chao[2] Wang, Deyin[3] Wang, Yucheng[2] Lu, Mengzhu[1]

第一作者：王留强

通信作者：Lu, MZ[1];Wang, YC[2]

机构：[1]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Key Lab Tree Genet & Breeding, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China;[3]Inst Jilin Forestry Invest & Design, Changchun 130022, Jilin, Peoples R China

年份：2017

卷号：60

期号：6

起止页码：548-557

外文期刊名：JOURNAL OF PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000417738000002)】；

基金：This work was supported by the Fundamental Research Funds of CAF (CAFYBB2017SY004) and National Natural Science Foundation of China (No. 31600539). We also thank the editor and reviewers for critical comments and thoughtful suggestions.

语种：英文

外文关键词：Oxidative stress; Peroxiredoxin; Reactive oxygen species; Tamarix hispida

摘要：Peroxiredoxins (Prxs) are ubiquitous thiol-specific antioxidant enzymes that are critically involved in cell defense and protect cells from oxidative damage. In this study, a putative Type II Prx (ThPrx1) was identified and characterized from Tamarix hispida. The expression of ThPrx1 is highly induced in response to hydrogen peroxide (H2O2) and methyl viologen (MV) stresses. When expressed ectopically, ThPrx1 showed enhanced tolerance against oxidative stress in yeast and Arabidopsis. In addition, transgenic Arabidopsis plants overexpressing ThPrx1 displayed improved seedling survival rates and increased root growth and fresh weight gain under H2O2 and MV treatments. Moreover, transgenic Arabidopsis plants showed decreased accumulation of H2O2, superoxide (O-2 (aEuro cent-)) and malondialdehyde (MDA), increased superoxide dismutase (SOD) activity compared to wild-type (WT) plants under oxidative stress. Moreover, transgenic plants maintained higher photosynthesis efficiency and lower electrolyte leakage rates than that of WT plants under stress conditions. These results clearly indicated that ThPrx1 plays an important role in cellular redox homeostasis under stress conditions, leading to the maintenance of membrane integrity and increased tolerance to oxidative stress.