文献类型：期刊文献

英文题名：VmUGT89AK4-mediated plant root resistance via regulating salicylic acid metabolism in tung trees

作者：Yang, Yang[1,2,3] Wang, Jia[1,2,3] Han, Fuchuan[1,2,3] Zhang, Jiantao[1,2,3] Liu, Beiping[1,2,3] Zhao, Yunxiao[1,2,3] Gao, Ming[1,2,3] Chen, Yicun[1,2,3] Wang, Yangdong[1,2,3]

第一作者：Yang, Yang

通信作者：Chen, YC[1];Wang, YD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Nanjing 210037, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2026

卷号：241

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20260620028206);WOS:【SCI-EXPANDED(收录号:WOS:001685182400001)】；

基金：This work was supported by the National Natural Science Foundation of China (32572095) and the Ten Thousand People Plan of Science and Technology Innovation Leading Talent of Zhejiang, China (No. 2022R52028) awarded to Yicun Chen.

语种：英文

外文关键词：Fusarium wilt disease; Tung tree; UGTs; Salicylic acid

摘要：Plants have evolved intricate defense mechanisms to combat pathogens infection. Maintaining a stable basal level of salicylic acid (SA) is crucial for plant growth and pathogens response, although basal levels of SA vary significantly among plant species. The SA and its sugar conjugates play important roles in maintaining metabolism. Vernicia, known as the tung oil tree, suffers from disease caused by F. oxysporum f. sp. fordiis (Fof-1), while its sister species Vernicia montana displays high resistance to Fof-1. Transcriptomic and metabolism analysis led to the discovery of four SA glycosyltransferase, including Vmo026507, Vmo021696 (VmUGT89AK4 Vmo018173, and Vmo003207 in V. montana. Here, based on the correlation coefficient (Pearson r) analysis tween the accumulation of SA metabolites and transcription levels of 4VmUGTs. The VmUGT89AK4 had highly significant correlation with root SAG (SA-glucoside) content expression. Enzyme activity assays revealed that VmUGT89AK4 exhibited catalytic activity on SA of substrates in vitro, form SAG. Transgenic tung tree over expression VmUGT89AK4 exhibited greater susceptibility to Fof-1 infection compared with EV plants, with OE-VmUGT89AK4 root accumulating significantly less SA level, and accompanied by reduced expression pathogen-related genes after Fof-1 infection. VmUGT89AK4-silenced tung tree exhibited greater resistance to Fof-1 infection than EV plants, and accumulated more SA and reduce SAG accumulation. These results suggest that VmUGT89AK4 is a SA glucosyltransferase that plays a negative regulatory factor by modulating SAG formation tung tree. Our findings reveal the role of VmUGT89AK4 in SAG formation and regulation of plant defense. While also shedding light on the role of a new subfamily SA glycosyltransferase (UGT89s), which offered a new progress into the resistance response of tung trees.