文献类型：期刊文献

中文题名：苏柳172和垂柳对Cu^(2+)的吸收特性及有机酸影响

英文题名：Uptake kinetic characteristics of Cu^(2+) by Salix jiangsuensis CL J-172 and Salix babylonica Linn and the influence of organic acids

作者：陈彩虹[1] 刘治昆[1,2] 陈光才[2] 单奇华[2] 张建锋[2]

第一作者：陈彩虹

机构：[1]中南林业科技大学;[2]中国林业科学研究院亚热带林业研究所

年份：2011

卷号：31

期号：18

起止页码：5255-5263

中文期刊名：生态学报

外文期刊名：Acta Ecologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：中央级公益性科研院所基本科研业务费专项资金重点项目(RISF6803);江苏省科技支撑计划项目(BE2009603;BE2008636)

语种：中文

中文关键词：苏柳172;垂柳;Cu^2+;吸收;有机酸

外文关键词：Salix jiangsuensis CL J-172; Salix babylonica Linn; Cu^2＋; uptake; organic acid

分类号：S945.1

摘要：采用溶液培养的方法,研究了苏柳172和垂柳对Cu2+吸收的动力学特性及低分子量有机酸(乙酸和苹果酸)对Cu2+吸收的影响。结果表明:苏柳172和垂柳根系对Cu2+的吸收量随溶液Cu2+浓度的升高及吸收时间的延长而增加。苏柳172和垂柳对Cu2+吸收的浓度动力学曲线呈线性,非饱和曲线,可以用米氏方程很好地拟合。米氏方程拟合的结果说明,垂柳根系的Cu2+吸收能力要稍强于苏柳172。Cu2+浓度为20μmol/L时,随着乙酸和苹果酸浓度的增加,对苏柳172和垂柳根系吸收Cu2+的促进作用呈先上升后下降的趋势,其中,乙酸在10∶1(乙酸∶Cu2+)、苹果酸在5∶1(苹果酸∶Cu2+)时促进作用最强。Cu2+在根尖区的含量远远大于成熟区,说明根尖区是吸收Cu2+最活跃的区域。在Cu2+浓度为20μmol/L的吸收液中加入乙酸或苹果酸(有机酸∶Cu2+=10∶1)时,苏柳172根尖区的Cu2+含量分别增加了16.5%和33.7%,根成熟区的Cu2+含量增加了23.7%和43.0%,但根尖区和成熟区的Cu2+含量比值分别是1.40和1.39,略小于不加有机酸时的1.49。乙酸与Cu2+的比例为10∶1时,当Cu2+浓度小于20μmol/L时乙酸促进垂柳根系对Cu2+的吸收,当Cu2+浓度大于20μmol/L时,乙酸抑制垂柳根系对Cu2+的吸收,抑制作用随Cu2+浓度的升高而加强,说明低浓度的乙酸可以促进垂柳根对Cu2+的吸收,而高浓度的乙酸能够抑制垂柳根吸收Cu2+。同时,乙酸抑制了Cu2+在垂柳地上部的积累,抑制作用随着Cu2+浓度的增加而加强,说明乙酸能够抑制Cu2+由根向地上部的运输。
Willow（Salix sp.） has large biomass production and high resistance to environmental stress as an important multipurpose tree species in afforestation,hence has been proved to be effective in the uptake and accumulation of metals from contaminated waters and soils.Salix jiangsuensis CL J-172 and Salix babylonica Linn were chosen as model plants to investigate their potential for uptake of Cu^2＋ from hydroponics culture solution. The uptake kinetic characteristics of Salix jiangsuensis CL J-172 and Salix babylonica Linn to Cu^2＋ and the effect of organic acids including acetic and malic acids to the Cu^2＋ accumulation by both willows species were investigated under hydroponics.The results suggested that the uptake influx of Cu^2＋ by roots of Salix jiangsuensis CL J-172 and Salix babylonica Linn increased with Cu^2＋ concentration varied from 0 to 200 μmol/L and the time prolonged from 0 to 48 hours.Concentration-dependent Cu^2＋ influx kinetics in Salix jiangsuensis CL J-172 and Salix babylonica Linn were characterized by smooth,non-saturating curves.The analysis of parameters derived from Michaelis-Menten equation showed that the absorption ability of Cu^2＋ by the root of Salix babylonica Linn was better than that of Salix jiangsuensis CL J-172.The addition of acetic acid enhanced the uptake of Cu^2＋ in roots and reached the highest values when the ratios of acetic acid to Cu^2＋ is 10：1,which increased the uptake of Cu^2＋ by 43.1% and 22.9% for Salix jiangsuensis CL J-172 and Salix babylonica Linn,respectively.The addition of malic acid enhanced the uptake of Cu^2＋ in roots and reached the highest values when the ratios of acetic acid to Cu2＋ is 5：1,which increased the uptake of Cu^2＋ by 61% and 9.2% for Salix jiangsuensis CL J-172 and Salix babylonica Linn,respectively.The content of Cu^2＋ in root cap was significantly higher than that in root manure zone in Salix jiangsuensis CL J-172 which suggest that root cap was more active in uptake of Cu^2＋ from solution.The addition of acetic acid increased the concentration of Cu^2＋ by 16.5% in root tip and by 23.7% in root mature zone,while malic acid increased by 33.7% in root tips and 43% in root mature zone at the ratio of organic acids to Cu^2＋ at 10∶1 for Salix jiangsuensis CL J-172,while the ratio of Cu^2＋ content in root cap to root mature zone changed slightly compared the value of 1.40 and 1.39 to the value of 1.49 without the addition of organic acid.With the ratio of acetic acid to Cu^2＋ at 10：1,Cu^2＋ uptake by root of Salix babylonica Linn were firstly enhanced when Cu^2＋ concentration were lower than 20 μmol/L and then decreased as Cu^2＋ concentration were higher than 20 μmol/L.The addition of acetic acid always decreased the content of Cu^2＋ in aboveground part of Salix babylonica Linn which implied that acetic acid inhibited the translocation of Cu^2＋ form root to aboveground part of Salix babylonica Linn and the inhibiting effect would be more obvious as the concentration of acetic acid increased.