文献类型：期刊文献

英文题名：Genetic Analysis of the Cultivars of Ping'ou Hybrid Hazelnut (C. heterophylla Fisch. x C. avellana L.) in China Based on SSR Markers

作者：Yang, Zhen[1] Zhao, Tiantian[1] Liang, Lisong[1] Jiang, Lei[2] Wang, Lujun[3] Hou, Sihao[1] Ma, Qinghua[1]

第一作者：杨振

通信作者：Ma, QH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry & G, Beijing 100091, Peoples R China;[2]Langfang Acad Agr & Forestry Sci, Langfang 065000, Peoples R China;[3]Anhui Acad Forestry, Hefei 230031, Peoples R China

年份：2023

卷号：14

期号：7

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001038675800001)】；

基金：This research was funded by the National Key R & D Program of China (2022YFD2200400), the Key Research and Development Program of Hebei Province (21326804D) and the Key Science and Technology Program of Anhui Province (202103B06020017).

语种：英文

外文关键词：Ping'ou hybrid hazelnut; SSR markers; germplasm identification; fingerprinting; genetic relationships

摘要：Ping'ou hybrid hazelnut is one of the most profitable tree nuts in China, but economically important cultivars must first be genetically validated to meet industrial demand. Traditional approaches used for cultivar identification are mainly trait-based and unreliable. Previous approaches at the DNA level, focusing on the identification of species or/and varieties that originated in China, were not used widely in hybrid hazelnut because there was no proper standard sample. In this research, a multiplexed fingerprinting test was conducted to allow for hazelnut cultivar identification using SSR markers derived from European hazelnut. Twenty-seven SSR markers were used to fingerprint 57 genetically unique Ping'ou hybrid hazelnut and related wild species. All markers showed a high level of polymorphism, as indicated by mean values for observed heterozygosity (H-o = 0.84), expected heterozygosity (H-e = 0.80), and polymorphism information content (PIC = 0.78). A total of 301 alleles were detected, and the number of effective alleles varied from 6 for KG817 and GB818 to 18 for B654, with an average of 11.2 alleles per locus. Moreover, the Shannon's information index (I) ranged from 1.293 for BR215 to 2.385 for B654, with an average of 1.908. The neighbor-joining tree, principal coordinate analysis, and Bayesian analysis revealed clear separation between hybrid cultivars and wild forms (Cluster/group I), as well as the differentiation within hybrid genotypes (Clusters/groups II and III). Additionally, the NJ dendrogram demonstrated a further split within Clusters/group III (III a and III b). Altogether, with the comparable SSR information of the European hazelnut cultivar 'Barcelona', the newly developed marker sets can assist in the germplasm identification of hazelnut cultivars and reproductive materials. Importantly, these combined SSR loci can be applied to characterize the genetic relationships and population structures among wild genotypes and hybrid cultivars, which will then provide information to guide hazelnut breeding based on their genetic background.