文献类型：期刊文献

英文题名：Expression of the SPL-like gene LaSPL9 in Japanese larch (Larix leptolepis) is regulated by miR156 during somatic embryogenesis

作者：Zhang, Li-feng[1] Fan, Yan-ru[1] Lan, Qian[2] Qi, Li-wang[1] Han, Su-ying[3]

第一作者：张立峰

通信作者：Zhang, Li-feng|[a000526fa064d5a9591a4]张立峰;

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing, 100091, China; [3] Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China

年份：2021

卷号：35

期号：5

起止页码：1727-1737

外文期刊名：Trees - Structure and Function

收录：EI(收录号：20210309778038);Scopus(收录号：2-s2.0-85099308184)

语种：英文

外文关键词：Proteins - Animal cell culture - Transcription - Encoding (symbols)

摘要：Key message: The Larix leptolepis SPL-like gene LaSPL9 was identified. Additionally, premiR156b and LaSPL9 expression patterns during somatic embryogenesis and the effects of gibberellin and salicylic acid on their transcription revealed the post-transcriptional regulatory functions of the miR156–LaSPL9 module. Abstract: A full-length cDNA sequence of a SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) gene was isolated from Japanese larch (Larix leptolepis) and designated as LaSPL9. The cDNA comprised 2735?bp, including a 1779-bp open reading frame encoding a protein (592 amino acids) with conserved SBP domains. The LaSPL9 promoter region included several gibberellin (GA)- and salicylic acid (SA)-inducible elements. Further analyses revealed that LaSPL9 mRNA is targeted by miR156. An examination of the LaSPL9-GFP fusion protein in tobacco leaf epidermal cells indicated that LaSPL9 is a nuclear protein. Additionally, LaSPL9 expression was induced by GA and the miR156b precursor (premiR156b) transcript accumulation was inhibited by SA. Moreover, abscisic acid (ABA) was identified as the main factor down-regulating LaSPL9 expression during the early stage of somatic embryogenesis (SE), which can induce premiR156b expression. Furthermore, LaSPL9 transcription peaked at 10?days, implying it might encode an important regulator of early embryonic pattern formation. The LaSPL9 transcript level subsequently decreased gradually and was lowest at 42?days, which is consistent with the gradual increase in miR156 expression during SE. The miR156–LaSPL9 module might regulate SE in L. leptolepis by responding to ABA. Overall, our results provide evidence that miR156–LaSPL9 is important for the crosstalk between GA and SA pathways during SE. ? 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.