文献类型：期刊文献

英文题名：Community structure and environmental determinants of the bacterial and fungal gut microflora in Hainan gibbons (Nomascus hainanus)

作者：Yang, Liangliang[1] Wang, Wenxia[2] Wronski, Torsten[3] Sun, Ping[4] Jin, Kun[5,6] Tang, Weilu[7]

通信作者：Jin, K[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry Policy & Informat, Beijing, Peoples R China;[3]Liverpool John Moores Univ, Fac Sci, Sch Biol & Environm Sci, Liverpool L3 3AF, England;[4]Eberswalde Univ Sustainable Dev, Fac Forest & Environm, D-16225 Eberswalde, Germany;[5]Chinese Acad Forestry, Res Inst Nat Protected Area, Beijing 100091, Peoples R China;[6]Grassland Adm, Key Lab Forest Protect Natl Forestry, Beijing 100091, Peoples R China;[7]Grassland Adm, East China Inventory & Planning Inst Natl Forestry, Hangzhou 310019, Peoples R China

年份：2022

卷号：36

外文期刊名：GLOBAL ECOLOGY AND CONSERVATION

收录：;Scopus(收录号：2-s2.0-85127914171);WOS:【SCI-EXPANDED(收录号:WOS:000798745900003)】；

基金：This work was funded by the key project of the Basic Scientific Research Business Expenses of the Central Public Welfare Scientific Research Institutes of the Chinese Academy of Forestry titled "Monitoring of key factors for the growth of Hainan gibbon population" (CAFYBB2019ZB010) and "Health Status Monitory of Hainan Gibbon Population", and National Forestry and Grassland Administration Wildlife Conservation Management Project "Delineation Standards for Important Wildlife Habitats and Directory" (21302112019-4017) .

语种：英文

外文关键词：Bacterial microbiome; Fungal microbiome; Hainan gibbon; Environmental determinants; Conservation

摘要：High-throughput sequencing technology was used to establish the OTU (Operational Taxonomic Units) composition and diversity of bacteria and fungi in the gut of three family groups of Hainan gibbons (Nomascus hainanus) in the Bawangling National Nature Reserve on Hainan Island, southern China. Firmicutes (47.23%) and Bacteroidetes (36.54%) predominated at the phylum level, while the predominant genera were Prevotella-7 (15.30%), Lachnospiraceae_NK3A20_group (12.49%), and uncultured_bacterium_f_Erysipelotrichaceae (11.79%). At the phylum level, the dominant fungi were Ascomycota (66.41%), as well as Hanseniaspora (23.33%), Schwanniomyces (13.44%), and Pichia (5.43%) at the genus level. We found significant differences in the bacterial OTU diversity (Shannon index) between family groups living in Tropical Lowland or Tropical Montane Rain Forest, compared to those inhabiting Tropical Montane Evergreen Forest at higher altitudes. Bacterial OTU community composition also differed between family groups, unraveling significant differences among the 30 most dominant bacterial core taxa. Furthermore, we unraveled a significant difference of OTU richness in the fungal microbiome (Chao 1) between family groups living at lower altitudes, i.e., Tropical Lowland or Tropical Montane Rain Forest and those inhabiting the Tropical Montane Evergreen Forest. Our analysis further indicated significant differences in the fungal OTU community composition between the three family groups, especially regarding the three most dominant fungal core taxa. Subsequently, two habitat factors, and nine environmental and anthropogenic variables were used to explore possible causes of disparity in the microbial flora of gibbon groups. A factor reduction procedure resulted into three principal components which were correlated to bacterial and fungal OTU richness and diversity using Spearman's rank-order correlations. Bacterial and fungal OTU diversity was high in areas of high altitude, steep slopes, high tree density, but low tree height, while high fungal OTU richness corresponded to high altitude habitats, i.e., in the Tropical Montane Evergreen Forest. Distance to human settlements and to the next water body showed no significant relation with bacterial and fungal richness and diversity.