文献类型：期刊文献

英文题名：Overexpression of geranyl diphosphate synthase small subunit 1 (LcGPPS.SSU1) enhances the monoterpene content and biomass

作者：Zhao, Yunxiao[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Yin, Hengfu[1,2] Wu, Liwen[1,2] Wang, Yangdong[1,2]

第一作者：赵耘霄

通信作者：Wu, LW[1];Wang, YD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2020

卷号：143

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000503323200001)】；

基金：This work was supported by Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFEBB2019ZX002). I am highly thankful to Dr. Zhibin Luo for his helpful suggestions.

语种：英文

外文关键词：Litsea cubeba; Geranyl diphosphate synthase small subunit (GPPS.SSU); Monoterpene; Tobacco; Feedback regulation; Biomass

摘要：Litsea cubeba (Lour.) Person, an economically important aromatic plant producing essential oils, has lemon-like fragrance and 96.44-98.44% monoterpene contents. Understanding the rate-limiting enzymes in the biosynthesis of monoterpenes in L cubeba serves the purpose of designing of new approaches to metabolic engineering. In this study, we investigated the function of gercmyi diphosphate synthase small subunit 1 (LcGPPS.SSU1), a branchpoint gene of monoterpenes biosynthesis pathway in L. cubeba. The expression analysis showed that LcGPPS.SSU1 was highly expressed in fruits, where the essential oil was produced and stored, and induced by light and methyl jasmonate (MeJA). In addition, the promoter of LcGPPS.SSU1 could drive the trichome-specific expression in tobacco. Overexpression of LeGPPS.SSU1 enhanced the monoterpene production in L. cubeba and tobacco (Nicotiana benthamiana). Moreover, analysing the expression level analysis of the key genes, especially 1-deoxy-D-xylulose 5- phosphate synthase (DXS), involved in mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathway showed the metabolic flux was increased by positive feedback regulation mechanism in LcGPPS.SSU1-overexpressing lines. Meanwhile, when MEP pathway flux increased, the seed yield, leaf area, plant height of LcGPPS.SSU1-overexpressing lines were enhanced by elevating the MEP-derived primary metabolites, such as gibberellin (GA), due to the increased expression levels of gerortylgeranyl diphosphate synthases 2 (GGPPS2), GGPPS4 and GA20-oxidase (GA20ox). Taken together, our results suggest that LeGPPS.SSUI plays a vital role in monoterpenes biosynthesis.