文献类型：期刊文献

英文题名：Callus induction and plant regeneration from anthers of Dendrocalamus latiflorus Munro

作者：Qiao, Guirong[1,2] Li, Haiying[1,2] Liu, Mingying[1,2] Jiang, Jing[1,2] Yin, Yafang[3] Zhang, Ling[1] Zhuo, Renying[1,2]

第一作者：乔桂荣

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Genom, Fuyang 311400, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China

年份：2013

卷号：49

期号：4

起止页码：375-382

外文期刊名：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000323515500001)】；

基金：The authors thank Professor Zongxiu Sun at the National Key lab of Rice Biology, Chinese National Rice Research Institute for technical advice. This work was supported by National Natural Science Foundation of China (31200508), Zhejiang Public Technology Research (2012C22099), Science Foundation of Zhejiang province (2010C12010), and National Non-profit Research Institute of CAF (CAFYBB2010003-1).

语种：英文

外文关键词：Bamboo; Plant growth regulator; Anther culture; Ploidy; Chromosome doubling

摘要：Bamboo varieties are very difficult to improve by traditional breeding methods. Here, we established an efficient plant-regeneration system for Dendrocalamus latiflorus (tropical giant bamboo) by anther culture. Culture conditions, especially the plant growth regulators required for callus induction and shoot differentiation, were optimized by orthogonal design. M8 medium supplemented with 5.37 mu Ie alpha-naphthaleneacetic acid (NAA), 1.33 mu M N (6) -benzyladenine (BA), 110.17 mu M phenylacetic acid (PAA), and a pretreatment time of 3 d produced the highest rate (5.08 +/- 0.61%) of callus induction. The maximum shoot differentiation rate reached 28.3 +/- 4.29% in M8 medium supplemented with 2.32 mu M kinetin (KT), 8.89 mu M BA, 1.08 mu M NAA, and 110.17 mu M PAA. The results of the ploidy level test showed that most of the regenerated plants were dodecaploid (96/100), a few were hexaploid (3/100), and one was triploid (1/100). The average chlorophyll content of dodecaploid lines was significantly higher than that of hexaploid lines. The present study provides an innovative method for bamboo ploidy breeding and a useful method for genetic improvement.