文献类型：期刊文献

英文题名：cDNA cloning, genomic organization and expression analysis during somatic embryogenesis of the translationally controlled tumor protein (TCTP) gene from Japanese larch (Larix leptolepis)

作者：Zhang, Li-feng[1] Li, Wan-feng[1] Han, Su-ying[2] Yang, Wen-hua[1] Qi, Li-wang[1]

第一作者：张立峰

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China

年份：2013

卷号：529

期号：1

起止页码：150-158

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000324669200024)】；

基金：This work was supported by the National Basic Research Program of China (2009CB119100), the National Natural Science Foundation of China (30830086), and the National High Technology Research and Development Program of China (2011AA100203, 2013AA102704).

语种：英文

外文关键词：Larix leptolepis; Somatic embryogenesis; Translationally controlled tumor protein; Genomic organization; Real-time quantitative PCR

摘要：A full-length cDNA and genomic sequences of a translationally controlled tumor protein (TCTP) gene were isolated from Japanese larch (Larix leptolepis) and designated LaTCTP. The length of the cDNA was 1043 bp and contained a 504 bp open reading frame that encodes a predicted protein of 167 amino acids, characterized by two signature sequences of the TCTP protein family. Analysis of the LaTCTP gene structure indicated four introns and five exons, and it is the largest of all currently known TCTP genes in plants. The 5'-flanking promoter region of LaTCTP was cloned using an improved TAIL-PCR technique. In this region we identified many important potential cis-acting elements, such as a Box-W1 (fungal elicitor responsive element), a CAT-box (cis-acting regulatory element related to meristem expression), a CGTCA-motif (cis-acting regulatory element involved in MeJA-responsiveness), a GT1-motif (light responsive element), a Skn-1-motif (cis-acting regulatory element required for endosperm expression) and a TGA-element (auxin-responsive element), suggesting that expression of LaTCTP is highly regulated. Expression analysis demonstrated ubiquitous localization of LaTCTP mRNA in the roots, stems and needles, high mRNA levels in the embryonal-suspensor mass (ESM), browning embryogenic cultures and mature somatic embryos, and low levels of mRNA at day five during somatic embryogenesis. We suggest that LaTCTP might participate in the regulation of somatic embryo development. These results provide a theoretical basis for understanding the molecular regulatory mechanism of LaTCTP and lay the foundation for artificial regulation of somatic embryogenesis. (C) 2013 Published by Elsevier B.V.