文献类型：期刊文献

英文题名：Transcriptome profiling reveals the roles of pigment formation mechanisms in yellow Paeonia delavayi flowers

作者：Zou, Hongzhu[1] Zhou, Lin[1] Han, Lulu[1] Lv, JiHang[1] Jia, YingHua[1] Wang, Yan[1]

第一作者：Zou, Hongzhu

通信作者：Wang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 10091, Peoples R China

年份：0

外文期刊名：MOLECULAR GENETICS AND GENOMICS

收录：;Scopus(收录号：2-s2.0-85145166088);WOS:【SCI-EXPANDED(收录号:WOS:000905236500001)】；

基金：AcknowledgementsThis work was supported by grants from the National Key Research and Development Program (Grant No. 2018YFD1000405) and The National Natural Science Foundation (Grant No. 31972456).

语种：英文

外文关键词：Transcriptome; Carotenoid metabolic pathway; Flavonoid metabolic pathway; Flower colour; Paeonia delavayi var; Lutea

摘要：The yellow colour of ornamental varieties of tree peony originated from Paeonia delavayi. However, but P. delavayi and Paeonia suffruticosa belong to different subgroups, so hybridization is difficult and results in a long breeding cycle. However, no comprehensive transcriptomic profiling has focused on the colour formation mechanisms of yellow tree peony petals. Analysing the colour formation mechanism of yellow petals in P. delavayi is very important for directional molecular breeding. In this study, the transcriptional map of yellow pigment development in petals was used to analyse the mechanism of petal colour formation. We analysed the genes related to the metabolism of flavonoids and carotenoids and the transcription factors (TFs) involved in P. delavayi var. lutea (pure yellow individual) yellow pigment development using transcriptome sequence profiling. Transcriptome sequence profiles revealed three and four differentially expressed transcripts (DETs) involved in flavonoid biosynthesis and carotenoid biosynthesis, respectively. An analysis of DETs in the flavonoid pathway showed that chalcone synthase (CHS) and chalcone 2 & PRIME;-glucosyltransferases (THC2'GT) act in synergy to synthesize isosalipurposide (ISP). CHS and flavonol synthase (FLS) synergistically synthesize quercetin and kaempferol. DEG analysis of the carotenoid pathway revealed that phytoene synthase (PSY), carotenoid isomerase (CRTISO) and beta-carotene hydroxylases (CHYB) play a key role in regulating lutein formation, and carotenoid cleavage dioxygenase (CCD) plays an important role in the degradation of carotenoids. These two pathways may be regulated by TF families such as bHLH, ARF, and MYB. The results of the transient overexpression of genes showed that CHS and CHI are regulated by PdMYB2. In this study, the molecular mechanism of ISP synthesis was analysed in depth, and the complete metabolic pathway of carotenoids in Paeonia L. was reported for the first time. By studying the formation mechanism of yellow pigment in P. delavayi petals, a breeding strategy for improving flavonol and carotenoid contents and reducing anthocyanin synthesis by genetic engineering was suggested.