文献类型：期刊文献

中文题名：AtDME1化学诱导表达载体的构建及其瞬时表达

英文题名：Constructing A Chemical-inducible Expression Vector of AtDME1 and Its Transient Expression

作者：常英英[1] 高亚南[1] 梁立雄[1] 丁昌俊[1] 苏晓华[1] 张冰玉[1]

第一作者：常英英

机构：[1]国家林业局林木培育重点实验室(中国林业科学研究院林业研究所)

年份：2016

卷号：44

期号：2

起止页码：75-79

中文期刊名：东北林业大学学报

外文期刊名：Journal of Northeast Forestry University

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：林木遗传育种国家重点实验室基本科研业务费专项资金课题(TGB2013010);国家高技术研究发展计划(863计划)课题(2013AA102703)

语种：中文

中文关键词：pER8-DME1;17-β-雌二醇;化学诱导表达载体;瞬时表达

外文关键词：pER8-DME1; 17-β-estradiol; Chemical-inducible expression vector; Transient expression

分类号：Q782

摘要：以拟南芥去甲基化转移酶基因DME1为目的基因,采用酶切、连接和重组反应的方法构建了以17-β-雌二醇为诱导剂的植物表达载体p ER8-DME1。将其转入农杆菌LBA4404菌株中,通过烟草瞬时表达技术,对该载体的诱导表达特性进行了研究。q PCR结果表明,较低浓度的17-β-雌二醇处理能够有效调控p ER8-DME1中目的基因的表达,诱导处理后目的基因表达量逐渐升高,在12 h后达到最高,之后缓慢下降。该载体的成功构建为深入研究DNA去甲基化与植物的生殖发育调控奠定了基础。
Dethylansferase gene DME1 from Arabidopsis thaliala was used as the target gene,and digestion,ligation and recombination reaction methods were applied for constructing the plant expression vector pE R8-DME1,which could be induced by 17-β-estradiol. The vector was transferred to Agrobacterium tumefaciens LBA4404,and the inducible expression characteristics were verified by the transient expression system of Nicotiana tabacum. By qP CR,low concentration of 17-β-estradiol could be effective for the expression of the target gene in pE R8-DME1 vector. The expression level of DME1 gene gradually increased with 17-β-estradiol treating time and reached the highest level at 12 h.