文献类型：期刊文献

中文题名：不同植物生长调节剂添加处理对杉木体胚成熟的影响

英文题名：Effects of Different Plant Growth Regulators on the Somatic Embryo Maturation of Cunninghamia lanceolata

作者：吴夏雷[1] 孙宇涵[1] 胡瑞阳[1,2] 曹森[1] 冯玥[1] 徐金良[3] 郑会全[4] 胡德活[4] 李云[1]

第一作者：吴夏雷

机构：[1]北京林业大学生物科学与技术学院北京林业大学林木分子设计育种高精尖创新中心林木育种国家工程实验室林木花卉遗传育种教育部重点实验室,北京100083;[2]中国林业科学研究院华北林业实验中心,北京102300;[3]浙江省开化县林场,开化324300;[4]广东省林业科学研究院,广州510520

年份：2019

卷号：17

期号：6

起止页码：2035-2041

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：广东省省级科技计划项目(2016B020201002);北京林业大学培育项目(2015ZCQ-SW-03; 2016BLPX13;2017CGP007)共同资助

语种：中文

中文关键词：杉木;体胚;成熟培养;谷胱甘肽

外文关键词：Cunninghamia lanceolata;Somatic embryo;Mature culture;Glutathione

分类号：S791.27

摘要：为优化杉木体细胞胚胎发生体系及探讨不同植物生长调节剂的添加处理对杉木体胚成熟的影响,本研究以继代中的杉木胚性愈伤组织为材料,使用不同浓度的脱落酸(ABA)与聚乙二醇-4000 (PEG-4000)组合添加剂和不同浓度的外源还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG)对成熟培养初期的杉木胚性愈伤组织进行处理,并对初次产生早期体细胞胚时的胚性愈伤组织进行谷胱甘肽过氧化物酶(GSH-PX)活性测定。结果显示,成熟培养在以DCR为基本培养基,附加蔗糖30 g/L、倍力凝5 g/L、活性炭1 g/L、硝酸银10 mg/L的条件下,同时添加ABA 50μmol/L和PEG-4000 100 g/L对杉木愈伤组织成熟具有明显的作用,成熟率达30.77%,其胚性愈伤组织大部分为白色透明、表面亮晶有光泽、质地松软粘连,表面具有明显伸长胚柄的早期体细胞胚再生出来,达到156.45个/g。在此基础上添加不同浓度的外源GSH和GSSG主要表现出低浓度促进,高浓度抑制的作用,其中添加GSH比GSSG影响更大,在GSH浓度为0.25 mmol/L时,成熟率最高,达到41.25%,比对照组高出12.50%,差异显著。本试验的研究发现对于优化杉木体胚成熟培养体系具有重要意义。
In order to optimize the somatic embryogenesis system and explore the effects of different plant growth regulators on somatic embryo maturation of Cunninghamia lanceolata, in this study, the embryonic callus of Cunninghamia lanceolata was used as a material. Different concentrations of abscisic acid(ABA) and polyethylene glycol-4000(PEG-4000) combination additives and different concentrations of exogenous reduced glutathione(GSH)and oxidized glutathione(GSSG) were used in the early stage of mature culture. The embryogenic callus of Cunninghamia lanceolata was treated, and the activity of glutathione peroxidase(GSH-PX) was determined for the embryogenic callus when the early somatic embryo was first produced. Results are as follows, mature culture was carried out with DCR as the basic medium(add sucrose 30 g/L, ploidy 5 g/L, activated carbon 1 g/L, silver nitrate 10 mg/L),adding ABA 50 μmol/L and PEG-4000 100 g/L has a significant effect on callus maturation, and the maturity rate is 30.77%. Most of the embryogenic callus was white and transparent, shiny on the surface, and the texture was soft and sticky. The early somatic embryos with apparently elongated suspensor on the surface were regenerated and reached 156.45 per gram. On this basis, the addition of different concentrations of exogenous GSH and GSSG mainly showing the effect of low concentration promotion and high concentration inhibition, in which the addition of GSH has greater influence than GSSG. When the concentration of GSH was 0.25 mmol/L, the maturation rate was the highest, reaching 41.25%, which was 12.50% higher than that of the control group, the difference was significant. The study found that this study has important significance for optimizing the mature culture system of Cunninghamia lanceolata.