文献类型：期刊文献

中文题名：2 种花楸果实类胡萝卜素成分与果色关系

英文题名：Relationship between Carotenoid Component and Fruit Color of Two Sorbus Species

作者：于雪丹[1] 张川红[1] 郑勇奇[1] 夏新合[1] 黄磊[2]

第一作者：于雪丹

机构：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091;[2]河北省小五台山国家级自然保护区管理中心,河北蔚县075700

年份：2021

卷号：34

期号：1

起止页码：71-79

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项资金项目“花楸树与北京花楸天然杂交的分子证据探究”(CAFYBB2017SY011)。

语种：中文

中文关键词：花楸树;北京花楸;果色;类胡萝卜素

外文关键词：Sorbus pohuashanensis;Sorbus discolor;fruit color;carotenoid

分类号：S792.25

摘要：[目的]对花楸树与北京花楸果实发育3个阶段类胡萝卜素成分进行代谢组学分析,为揭示花楸果实显色机理奠定基础。[方法]利用高效液相色谱串联质谱法对花楸树和北京花楸果实类胡萝卜素进行定性和定量检测,对类胡萝卜素成分和积累量的种内时序性差异和同时序的种间差异及其与果色的关系进行分析。[结果]定性分析表明:花楸树和北京花楸果实分别含12种和7种类胡萝卜素。定量分析表明:花楸树果实发育3个阶段类胡萝卜素积累量均显著高于北京花楸,花楸树类胡萝卜素积累量随果实发育时序变化显著升高。β-胡萝卜素是花楸树类胡萝卜素的主要成分,积累量从33.40 mg·kg^(?1)增至81.40 mg·kg^(?1),比例从54.68%增至79.36%。北京花楸果实中各种类胡萝卜素物质积累量均随果实发育显著降低。[结论]花楸树中类胡萝卜素主要成分为β-胡萝卜素,果实显色程度与其积累量增加显著正相关;北京花楸果实中类胡萝卜素积累量显著低于花楸树,并随果实发育时序性显著下降,是其果实为白色而不显其他颜色的主要原因之一。
[Objective]To analyze the metabolomic characteristics of carotenoid components in the fruits of Sorbus pohuashanensis and S.discolor in three developmental stages of fruit for the purpose of studying the mechanism of fruit coloration of Sorbus trees.[Method]The carotenoid components in fruits of the two Sorbus species were qualitatively and quantitatively determined by liquid chromatography tandem mass spectrometry(LC-MS/MS)to compare the differences between the two species,and to analyze the relationship between carotenoid component of fruit and the color of pericarp.[Result]Qualitative analysis showed there was a total of twelve carotenoids in S.pohuashanensis and seven in S.discolor.Quantitative analysis indicated that carotenoid accumulation of S.pohuashanensis were higher significantly than that of S.discolor at three developmental stages of fruit.As the fruit ripening,the carotenoid accumulation of S.pohuashanensis increased significantly,β-carotene was the main component,the accumulation ofβ-carotene increased from 33.40 mg·kg^(?1) to 81.40 mg·kg^(?1),and the percentage increased from 54.68%to 79.36%,while the carotenoid accumulation of S.discolor decreased significantly during fruit development.[Conclusion]β-carotene is the main carotenoid compound in the fruit of S.pohuashanensis,and the coloration of fruit was significantly and positively correlated with the accumulation ofβ-carotene.The accumulation of carotenoid in S.discolor is significantly lower compared with S.pohuashanensis,and the carotenoid content reduces significantly within the development process which is one of the factors leading to white pericarp of the fruit without other colors.