文献类型：期刊文献

英文题名：Chloroplast Genomes of Genus Tilia: Comparative Genomics and Molecular Evolution

作者：Yan, Linjun[1] Wang, Huanli[1] Huang, Xi[1] Li, Yingchao[2] Yue, Yuanhao[1] Wang, Zhongwei[1] Tang, Shijie[1]

第一作者：Yan, Linjun

通信作者：Tang, SJ[1]

机构：[1]Jiangsu Prov & Chinese Acad Sci, Jiangsu Key Lab Res & Utilizat Plant Resources, Inst Bot, Nanjing Bot Garden, Nanjing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000830184900001)】；

基金：Funding This work was supported by the Natural Science Foundation of Jiangsu Province under Grant Number (BK20170619), National Natural Science Foundation of China under Grant Number (31700477), and Innovation and Extension of Forestry Science and Technology of Jiangsu Province under Grant Number [LYKJ(2019)06].

语种：英文

外文关键词：Malvaceae; chloroplast genome; hypervariable regions; comparison analysis; phylogenetic analysis

摘要：Tilia is a complex genus in the family Malvaceae that has high ecological and economical values. Owing to the lack of sufficient distinguishable morphological and molecular characteristics, interspecific relationships in this genus are not clear. Chloroplast (cp) genomes are small, meanwhile most angiosperms usually undergo matrilineal inheritance. Consequently, they can be used in molecular systematics and phylogenetic analyses. Here, we sequenced and assembled cp genomes from T. endochrysea, T. tomentosa, T. miqueliana, T. americana and T. cordata, and compared them with those of seven previously reported Tilia species. Similar gene contents, gene orders and GC contents existed among the 12 cp genomes, which ranged from 162,564 to 162,855 bp and encoded 113 unique genes. Abundant simple sequence repeats (119-127) and dispersed repeats (97-135) were detected in Tilia cp genomes. In total, 11 hypervariable regions were identified that could be suitable for species identification and phylogenetic studies. A phylogenetic analysis of Malvaceae based on 5 hypervariable genes (matK + ndhF + rpoB + rpoC2+ycf1) revealed that all eight subfamilies were monophyletic groups. Additionally, the genus Tilia was divided into three groups on the basis of all 521 molecular variation loci. The current study provides valuable insights into the genomic evolution of the genus Tilia.