文献类型：期刊文献

英文题名：Two terpene synthases in resistantPinus massonianacontribute to defence againstBursaphelenchus xylophilus

作者：Liu, Bin[1,2] Liu, Qinghua[1,2] Zhou, Zhichun[1,2] Yin, Hengfu[1] Xie, Yini[1,2] Wei, Yongcheng[3]

第一作者：Liu, Bin

通信作者：Liu, QH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou, Peoples R China;[3]Chinese Acad Forestry, Inst Trop Forestry, Guangzhou, Peoples R China

年份：2021

卷号：44

期号：1

起止页码：257-274

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000574179900001)】；

基金：Major Special Project on Breeding New Forest Trees in Zhejiang Province of China during the Thirteenth Five-plan Period, Grant/Award Number: 2016C02056-4; National Natural Science Foundation of China, Grant/Award Number: 31470670; Zhejiang Science and Technology Program, Grant/Award Number: 2020C02007

语种：英文

外文关键词：bifunctional enzyme; defence mechanism; pine wood nematode; resistance; signal-specific expression

摘要：Pine wood nematode (PWN;Bursaphelenchus xylophilus), a destructive pest ofPinus massoniana, is causing a severe epidemic of pine wilt disease in China. When invaded by PWN, resistantP. massonianasecretes an abundance of oleoresin terpenoids as a defensive strategy. However, regulatory mechanisms of this defence in resistantP. massonianahave yet to be elucidated. Here, we characterized two terpene synthase genes,alpha-pinene synthase(PmTPS4) andlongifolene synthase(PmTPS21), identified in resistantP. massonianaand investigate the contribution of these genes to the oleoresin defence strategy in resistant masson pines. Up-regulation of these two genes in the stem supported their involvement in terpene biosynthesis as part of the defence against PWN. Recombinant protein expression revealed catalytic activity for the two PmTPSs, with PmTPS4 primarily producing alpha-pinene, while PmTPS21 produced alpha-pinene and longifolene simultaneously. The major enzymatic products of the two terpene synthases had inhibitory effects on PWN in vitro. We demonstrated thatPmTPS4andPmTPS21played positive roles in terpene-defence mechanisms against PWN infestation. The major products of these terpene synthases could directly inhibit the survival rate of PWN in vitro. We revealed that PmTPS21 was a novel bifunctional enzyme capable of simultaneous production of both monoterpene and sesquiterpene.