文献类型：期刊文献

英文题名：Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages

作者：Zhan, Zhiyong[1,2,3] Chen, Yicun[1,2] Shockey, Jay[4] Han, Xiaojiao[1,2] Wang, Yangdong[1,2]

第一作者：Zhan, Zhiyong

通信作者：Wang, YD[1];Wang, YD[2]|[a0005f34f1ecf07bb8f24]汪阳东;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Foresty, Fuyang 311400, Zhejiang, Peoples R China;[3]Jiangxi Acad Forestry, Nanchang 330013, Jiangxi, Peoples R China;[4]USDA ARS, Southern Reg Res Ctr, New Orleans, LA 70124 USA

年份：2016

卷号：21

期号：11

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000389918200068)】；

基金：This work was supported financially by Zhejiang Province Natural Science Foundation of China (LZ13C160001).

语种：英文

外文关键词：tung tree; seed; oil content; oil body; development; proteomics

摘要：The tung tree (Vernicia fordii), a non-model woody plant belonging to the Euphorbiaceae family, is a promising economic plant due to the high content of a novel high-value oil in its seeds. Many metabolic pathways are active during seed development. Oil (triacylglycerols (TAGs)) accumulates in oil bodies distributed in the endosperm cells of tung tree seeds. The relationship between oil bodies and oil content during tung tree seed development was analyzed using ultrastructural observations, which confirmed that oil accumulation was correlated with the volumes and numbers of oil bodies in the endosperm cells during three different developmental stages. For a deeper understanding of seed development, we carried out proteomic analyses. At least 144 proteins were differentially expressed during three different developmental stages. A total of 76 proteins were successfully identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/ mass spectrometry (MALDI-TOF/MS/MS). These proteins were grouped into 11 classes according to their functions. The major groups of differentially expressed proteins were associated with energy metabolism (25%), fatty acid metabolism (15.79%) and defense (14.47%). These results strongly suggested that a very high percentage of gene expression in seed development is dedicated to the synthesis and accumulation of TAGs.