文献类型：期刊文献

英文题名：Effects of grazing on N2O production potential and abundance of nitrifying and denitrifying microbial communities in meadow-steppe grassland in northern China

作者：Zhong, Lei[1,2] Du, Rui[1] Ding, Kai[1] Kang, Xiaoming[3] Li, Frank Yonghong[2] Bowatte, Saman[2] Hoogendoorn, Coby J.[2] Wang, Yanfen[1,4] Rui, Yichao[1] Jiang, Lili[1] Wang, Shiping[5]

第一作者：Zhong, Lei

通信作者：Wang, Y.

机构：[1] University of Chinese Academy of Sciences, Beijing 100049, China; [2] AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North 4442, New Zealand; [3] Institute of Wetland Research, Chinese Academy of Forestry, Beijing 100091, China; [4] Research Network of Global Change Biology, Beijing Institutes of Life Science, The Chinese Academy of Sciences, Beijing, China; [5] Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100101, China

年份：2014

卷号：69

起止页码：1-10

外文期刊名：Soil Biology and Biochemistry

收录：EI(收录号：20134616988082);Scopus(收录号：2-s2.0-84887361578)

语种：英文

外文关键词：Nitrogen oxides - Bacteria - Lighting - Acetylene - Soil surveys - Nitrification - Ammonia - Denitrification - Genes - Polymerase chain reaction

摘要：Purpose: The aim of this study was to investigate the effects of cattle grazing on the nitrous oxide (N2O) production potential from meadow-steppe grassland soil in northern China, and the relationship between cattle grazing and the abundance of different functional microbial genes for potential of N2O emissions. Materials and methods: We collected soil samples at a depth of 0-20cm over six times during two plant growing seasons in 2011 and 2012 on a native Leymus chinensis grassland. At each of the six sampling occasions, soil samples were taken from three pairs of the cattle grazed vs. ungrazed plots. We then determined (1) the soil moisture, pH, total carbon and nitrogen, and mineral N (NH4+ and NO3-) content, (2) the potential rates of N2O production from nitrification (NN2O) and from denitrification (DN2O and DN2) using the acetylene inhibition method, and (3) the abundance of the amoA (ammonia monooxygenase) gene of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), the narG (nitrate reductase) gene and nosZ (nitrous oxide reductase) gene using quantitative real-time PCR (qPCR). The relationship among the changes in the N2O production potential rates, the abundance of microbial functional groups and the soil environment was analyzed using mix effects modeling and structural equation modeling. Results and discussion: The AOA, AOB, narG, nosZ genes and the potential N2O production rate all varied significantly with the season (P4+ content and soil moisture; The abundance of narG gene, along with total C, NO3- content and soil moisture, explained 87% of variation in the DN2 (P+N2), but not the DN2O. Soil moisture was the best predictor for DN2O. Conclusions: The abundance of amoA and narG genes are good indicators for the potential nitrification and denitrification rates in the meadow steppe grassland. Soil moisture is the most important factor controlling the N2O emission potential in the meadow-steppe grassland. The grassland soils protected from animal grazing or that under a moderate grazing for five years did not show a significant difference in potential N2O emissions. Our results suggest that grazing induced grassland degradation may not necessarily be associated with a reduction in N2O emissions as reported in other semiarid grasslands in a more arid environment. ? 2013 Elsevier Ltd.