文献类型：期刊文献

中文题名：田间低温下多年生黑麦草再生株系的抗寒性研究

英文题名：Cold Resistance Research of Regenerated Lines of Lolium perenne Under Natural Low Temperature

作者：翟飞飞[1] 韩蕾[1] 李伟[1] 张晓丽[1] 巨关升[1] 孙振元[1]

第一作者：翟飞飞

机构：[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室

年份：2015

卷号：0

期号：5

起止页码：961-971

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"863"计划项目(2011AA100209)

语种：中文

中文关键词：多年生黑麦草;再生株系;抗寒性;酶活性;渗透调节;叶绿素荧光

外文关键词：perennial ryegrass, regenerated ines, cold resistance, enzyme activity, osmotic regulation, chlorophyll fluorescence

分类号：S688.4

摘要：为了获得抗寒性好的多年生黑麦草新种质,本研究以多年生黑麦草Derby的19个再生株系和抗寒性较好的Pinnacle为材料,连续2年进行越冬性观测,获得5个表现良好的再生株系。然后在田间低温胁迫下,测定与抗寒性相关的生理生化指标,以期对5个再生株系的抗寒性进行综合评价。越冬性调查结果显示,CK14-3和G16-1在越冬率、绿色期及分蘖速率等方面都表现较好。田间低温下,各试材的叶片含水量(LWC)降低;细胞膜受损程度加重:电导率(EC)和丙二醛(MDA)含量升高;渗透调节物质脯氨酸(Pro)、可溶性糖(SS)和还原性糖(RS)含量升高,可溶性蛋白(SP)含量下降;超氧化物歧化酶(SOD)活性升高,过氧化物酶(POD)和过氧化氢酶(CAT)活性下降;叶绿素(Chl)含量和潜在光化学效率(Fv/Fm)下降。经主成分与隶属函数分析,各试材的抗寒性从强到弱依次是Pinnacle、CK14-3、G16-1、CK9-3、G3-2、G10-5。采用聚类分析法把试材分为3类:Pinnacle和CK14-3的抗寒性强;G16-1和CK9-3的抗寒性中等;G3-2和G10-5的抗寒性差。综上,田间低温胁迫下抗寒性强的多年生黑麦草,细胞膜受伤害程度轻,Pro和RS含量低,SS含量和SOD活性增加率、POD活性下降率大,CAT活性、Chl含量和Fv/Fm下降率小;CK14-3和G16-1的越冬性表现良好、综合排名靠前,有望培育出抗寒性好的新种质。本研究不仅为诱变育种的抗逆性种质选育提供参考,还为多年生黑麦草的抗寒性研究提供理论基础。
To obtain new germplasm with good cold resistance, nineteen regenerated lines of perennial ryegrass from ＇Derby＇ and ＇Pinnacle＇ with good cold resistance were used in this research. After two winters＇ observation, five regenerated lines with good performance were obtained. Then some physiology and biochemistry indexes relating to cold resistance were measured under natural low temperature. The aim was to assess cold resistance of five lines comprehensively, screen new germplasm with excellent hardiness, and find out indexes relating to freezing tolerance. The survey of overwintering showed that CK14 - 3 and G16 - 1 behaved well in green period, tillering and overwinter rate. Under natural low temperature, leaf water content reduced and cytomembrane was injured. Proline, soluble sugar, and reducing sugar content increased, while soluble protein content decreased. The activity of superoxide dismutase increased, while the activities of peroxidase and catalase decreased. Moreover, photosynthetic system was impaired, both of chlorophyll content and potential photochemical efficiency decreased. By principal component analysis and membership function calculating, the cold-resistance ranking of perennial ryegrass was ＇Pinnacle＇ , CK14 -3, G16 - 1, CK9- 3, G3 -2, G10 -5 from strong to weak. The experiment materials could be classified into three types： ＇ Pinnacle＇ and CK14 - 3 were strong in cold-tolerance, G16 - 1 and CK9 - 3 were medium, and G3 - 2 and G10 - 5 were poor. The study indicates that the more cold-resistance the perennial ryegrass is, the less cytomembrane is damaged ; the less proline content and reducing sugar content it has, the quicker increasing rate of soluble sugar content and superoxide dismutase activity and reducing rate of peroxidase activity is, the slower reducing rate of catalase activity, chlorophyll content and potential photochemical efficiency change is. CK14 -3 and G16 - 1 perform well in cold hardiness and ranked in top, so they were probable to breed as new germplasm with good cold resistance. This study not only provides a reference for germplasm breeding of mutation with good resistance, but also offers theoretical basis for cold resistance research of perennial ryegrass.