文献类型：期刊文献

英文题名：Genome expression analysis of basic helix-loop-helix transcription factors in Sea buckthorn (Hippophae rhamnoides L.)

作者：Zhang, Jiajia[1,2] Du, Gaigai[2,3] Zhang, Guoyun[4] Zhang, Jianguo[4] Diao, Songfeng[2]

第一作者：Zhang, Jiajia

通信作者：Diao, SF[1]

机构：[1]Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Peoples R China;[2]Chinese Acad Forestry, Res Inst Nontimber Forestry, Key Lab Nontimber Forest Germplasm Enhancement & U, Natl Forestry & Grassland Adm, Zhengzhou, Peoples R China;[3]Radboud Univ Nijmegen, Nijmegen, Netherlands;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2024

卷号：15

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85211174558);WOS:【SCI-EXPANDED(收录号:WOS:001368813200001)】；

基金：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the National Key R&D Program of China (2023YFD2200303, 2022YFD2200401).

语种：英文

外文关键词：Hippophae rhamnoides; genome-wide analysis; basic helix-loop-helix TFs; expression analysis; fruit development

摘要：Introduction The basic helix-loop-helix (bHLH) transcription factor family is one of the largest gene families in plants, extensively involved in plant growth, organ development, and stress responses. However, limited studies of this family are available in sea buckthorn (Hippophae rhamnoides).Methods In this study, we identified 144 bHLH genes in H. rhamnoides (HrbHLH) through a genome-wide search method, then explored their DNA and protein sequences and physicochemical properties.Results and discussion According to the sequence similarities, we classified them into 15 groups with specific motif structures. To explore their expressions, we performed gene expression profiling using RNA-Seq and identified 122 HrbHLH mRNAs were highly expressed, while the remaining 22 HrbHLH genes were expressed at very low levels in all 21 samples. Among these HrbHLH genes, HrbHLH47, HrbHLH74, HrbHLH90, HrbHLH131 showed the highest expression level in the root nodule, root, leaf, stem and fruit tissues. Furthermore, eleven HrbHLH genes displayed increased expressions during the fruit development process of sea buckthorn. Finally, we validated the expression patterns of HrbHLH genes using reverse transcription quantitative real-time PCR (QPCR). This comprehensive analysis provides a useful esource that enables further investigation of the physiological roles and molecular functions of the HrbHLH TFs.