文献类型：期刊文献

中文题名：尾叶桉和细叶桉无性系的RAPD指纹图谱构建

英文题名：RAPD Fingerprints of Clones of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith

作者：甘四明[1] 施季森[1] 白嘉雨[2] 徐建民[2]

第一作者：甘四明

机构：[1]南京林业大学林木遗传和基因工程实验室;[2]中国林业科学研究院热带林业研究所

年份：1999

卷号：23

期号：1

起止页码：11-14

中文期刊名：南京林业大学学报：自然科学版

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：世行贷款造林项目;广东省桉树发展工程项目

语种：中文

中文关键词：尾叶桉;细叶桉;RAPD;指纹图谱

外文关键词：Eucalyptus urophylla S. T. Blake; Eucalyptus tereticornis Smith; RAPD; Fingerprint

分类号：S792.390.1

摘要：利用ＲＡＰＤ分子标记技术对来自９个起源地点的尾叶桉（ＥｕｃａｌｙｐｔｕｓｕｒｏｐｈｌａＳ．Ｔ．Ｂｌａｋｅ）和细叶桉（Ｅ．ｔｅｒｅｔｉｃｏｒｎｉｓＳｍｉｔｈ）共２２个无性系的研究表明，１３个引物共扩增出１１０个位点，多态性位点９１个，在起源地点间差异显著的多态性位点２５个，各无性系有其特异的ＲＡＰＤ扩增谱带。利用多态性位点的数据矩阵，计算了各无性系间的遗传距离，并构建了无性系间的遗传关系聚类图。
Fingerprints of 13 clones of 5 origins of Eucalyptus urophylla S. T. Blake and 9 clones of 4 origins of E. tereticornis Smith were constructed with RAPD markers. The 13 primers screened could result in 110 loci, of which 91 loci were polymorphic and 25 were different at 0.10 significance level among the origins. The genetic distances between clones were calculated, on the basis of which a dendrogram was established. The construction of such fingerprints could be of very importance for identification of clone, clarification of genetic relationship between clones and selection of parental combination in hybridization of the two species.