文献类型：期刊文献

英文题名：Chromosome-level genome assembly of Hippophae tibetana provides insights into high-altitude adaptation and flavonoid biosynthesis

作者：Zhang, Guoyun[1,2] Song, Yating[1,2] Chen, Ning[1,2] Wei, Jihua[1,2] Zhang, Jianguo[1,2,3] He, Caiyun[1,2]

第一作者：张国昀

通信作者：Zhang, JG[1];He, CY[1];Zhang, JG[2];He, CY[2];Zhang, JG[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing, Peoples R China

年份：2024

卷号：22

期号：1

外文期刊名：BMC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001201816100005)】；

基金：The platform of the facilities from the State Key Laboratory of Tree Genetics and Breeding is acknowledged. We thank Jiayi Li for her helpful suggestion.

语种：英文

外文关键词：Hippophae tibetana; Chromosome-level genome; Adaptation; Flavonoid biosynthesis

摘要：Background As an endemic shrub of the Qinghai-Tibetan Plateau (QTP), the distribution of Hippophae tibetana Schlecht. ranges between 2800 and 5200 m above sea level. As the most basal branch of the Hippophae genus, H. tibetana has an extensive evolutionary history. The H. tibetana is a valuable tree for studying the ecological evolution of species under extreme conditions. Results Here, we generated a high-quality chromosome-level genome of H. tibetana. The total size of the assembly genome is 917 Mb. The phylogenomic analysis of 1064 single-copy genes showed a divergence between 3.4 and 12.8 Mya for H. tibetana. Multiple gene families associated with DNA repair and disease resistance were significantly expanded in H. tibetana. We also identified many genes related to DNA repair with signs of positive selection. These results showed expansion and positive selection likely play important roles in H. tibetana's adaptation to comprehensive extreme environments in the QTP. A comprehensive genomic and transcriptomic analysis identified 49 genes involved in the flavonoid biosynthesis pathway in H. tibetana. We generated transgenic sea buckthorn hairy root producing high levels of flavonoid. Conclusions Taken together, this H. tibetana high-quality genome provides insights into the plant adaptation mechanisms of plant under extreme environments and lay foundation for the functional genomic research and molecular breeding of H. tibetana.