文献类型：期刊文献

英文题名：Mating patterns and pollen dispersal in a Japanese larch (Larix kaempferi) clonal seed orchard: a case study

作者：Chen, Xingbin[1,2,3] Sun, Xiaomei[1,2] Dong, Leiming[1,2] Zhang, Shougong[1,2]

第一作者：Chen, Xingbin

通信作者：Zhang, SG[1];Zhang, SG[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Jiangxi Acad Forestry, Jiangxi Prov Key Lab Plant Biotechnol, Nanchang 330013, Jiangxi, Peoples R China

年份：2018

卷号：61

期号：9

起止页码：1011-1023

外文期刊名：SCIENCE CHINA-LIFE SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000447777300003)】；

基金：This work was supported by the Forestry Industry Research Special Funds for Public Welfare Projects (201504104). We gratefully thank Wusheng Liu for his guidance and thorough review of the manuscript.

语种：英文

外文关键词：Larix kaempferi; simple sequence repeats (SSRs); paternity analysis; pollen contamination; pollen dispersal; growth performance

摘要：Pollination dynamics highly determines the genetic quality of seed orchard crops. However, there is less research about the effect of mating patterns on seed productivity of orchard crops. So far, clonal seed orchards have been producing genetically improved seedlings used for most Japanese larch (Larix kaempferi (Lamb.) Carr.) plantations in China. In the present study, a total of 17 highly variable simple sequence repeat (SSR) markers were used for genotyping a progeny trial population consisting of 647 open-pollinated progenies germinated from seeds which were collected from 63 maternal clones with 140 potential paternal clones in a Japanese larch clonal seed orchard in China. Paternity analysis was used in the present case study in order to evaluate the level of paternal gametic contribution, estimate pollen contamination and selfing rates, and investigate pollination patterns, pollen dispersal patterns and the impact of mating patterns on seed productivity of orchard crops. We observed 93.7% of the success rate of the parental assignment, unequal paternal gametic contribution (0-12.4%) with 6.3% of the progenies derived from pollen contamination or unsampled pollen donors, and absence of evidence for selfing. We also found that pollination rate highly depended on the distance between pollen donors and maternal parents, the majority of the identified crossing (65.7%) occurred between clones within a 150-m radius, and large variations in growth performance existed among the paternal half-siblings. Progeny growth performance (diameter at breast (DBH) and height (HGT)) was measured at Age-20 in order to investigate the impact of mating patterns on timber production of orchard crops. As either the paternal or maternal, two clones (i. e., clones Z38 and Z62) were identified to have produced progenies with higher average stem volume breeding values than that of all of the progenies. Specifically, the genetic gains for volume were 3.53% for the two clones as paternal parents, and 8.26% as the maternal parents at Age-20. Thus, both elite clones were ideal candidates for the construction of next-generation clonal seed orchards due to their synchronous reproductive phenology with greater crossing rate and higher genetic gain. These results improved the pedigree information to provide solid evidence of mating patterns for future design and effective management of seed orchards and for the development of viable long-term breeding strategies for other coniferous species.