文献类型：期刊文献

中文题名：转Bt基因欧洲黑杨抗虫稳定性及其对天敌昆虫的影响

英文题名：Stability of Insect-resistance of Bt Transformed Populus nigra Plantation and Its Effects on the Natural Enemies of Insects

作者：胡建军[1] 李淑梅[1] 卢孟柱[1] 李继祥[2] 李开花[2] 孙雪芹[2] 赵自玉[2]

第一作者：胡建军

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室;[2]新疆玛纳斯平原林场

年份：2007

卷号：20

期号：5

起止页码：656-659

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家转基因专项(J2002-B-003)

语种：中文

中文关键词：欧洲黑杨;Bt基因;抗虫稳定性;天敌昆虫;生物安全

外文关键词：Populus nigar ; Bt gene ; insect-resistance ; nature enemy ; biosafety

分类号：S722.3

摘要：1997—2001年,对新疆玛纳斯平原林场的转Bt基因欧洲黑杨试验林进行了食叶害虫(杨尺蠖)和杨梦尼夜蛾危害调查,转基因试验林叶片平均损失率均低于10%,试验林地虫口密度由1997年的18头.m-2降低到2001年的8头.m-2,而对照林地虫口密度却呈上升趋势。2005年,位于北京怀柔的转Bt基因欧洲黑杨试验林受杨尺蠖危害,叶片损失率不超过20%,而当地其它杨树林叶片损失率高达90%。在转Bt基因欧洲黑杨林(新疆玛纳斯)中,寄生靶标害虫的天敌种类、数量和寄生率均高于对照林,对从试验林中采集的靶标昆虫的蛹进行接种试验,与对照相比,寄生蜂出蜂率和数量均无明显差异,寄生蜂也无异常,说明转Bt基因欧洲黑杨未对天敌昆虫产生明显不利影响。
The damage caused by Apocheimia cinerarius Erschoff and Orthosia incerta Hufnage in Bt transformed Pop- ulus nigra plantation located in Manasi Plain Forest Station, Xinjiang Uygur Autonomous Region was investigated during 1997 -2001. The leaf loss was less than 10% and the larvae density in the soil decreased from 18 heads ·m^-2 in 1997 to 8 heads · m ^-2 in 2001, hut the larvae density increased in the non-transgenic plantation. In 2005, the leaf loss caused by Apocheimia cinerarius Erschoff in Bt transformed plantation in Huairou of Beijing was less than 20%, hut it was 90% in non-transgenic poplar plantation. This indicated that the insect-resistance of transgen- ic poplar was stable, and can reduce the larvae density effectively in the field for many years. The variety, number and parasitic ratio of the natural enemies of insects in the transgenic poplar plantation （Manasi） were more than hose in the non-transgenic poplar plantations. The result of the inoculation of the insect pupae collected from trans- genie poplar plantation, nearby poplar plantation and the control plantation with the wasp （ Chouioia cunea Yang. ） showed that there was no significant difference of＇ the wasp eclosion rate and number between them. It can be con- cluded that up to know there is no significant negative effect of transgenic poplar on the natural enemies of insects.