文献类型：期刊文献

中文题名：Fe^(3+)浓度对核桃成熟子叶离体生根培养的影响

英文题名：Effects of Fe^(3+) concentration on rooting culture of mature walnut cotyledon in vitro

作者：周贝贝[1] 张俊佩[1] 王伟[1] 马庆国[1] 裴东[1] 王少明[2] 郭志民[2]

第一作者：周贝贝

机构：[1]中国林业科学研究院林业研究所;[2]河南省国有洛宁县吕村林场

年份：2011

卷号：29

期号：1

起止页码：124-127

中文期刊名：经济林研究

外文期刊名：Nonwood Forest Research

收录：CSTPCD

基金：林业公益性行业科研专项(201004048;4-46)

语种：中文

中文关键词：核桃;子叶生根;铁盐

外文关键词：walnut; rooting of cotyledon; EDTA-Fe

分类号：S664.1

摘要：为给研究子叶生根的机理和铁盐作用机制提供参考,以"辽宁1号"核桃品种成熟子叶近胚端为材料,研究了Fe3+浓度对子叶不定根发生和伸长的影响。结果表明:当Fe3+浓度为0时生根能力最差,第12天生根率为66%,随着Fe3+浓度升高,生根能力明显增强,以0.352μmol/L为最优,第8天生根率为100%。Fe3+浓度继续升高,从0.704μmol/L开始子叶接近培养基部分出现褐化现象,7.04μmol/L时,褐化最严重,第12天褐化率达到50%,生根能力下降,第12天生根率为95%。
In order to provide reference to the studies on cotyledon rooting and Fe3＋ process mechanisms,taking Liaoning No.1 walnut cotyledon as materials,effects of different Fe3＋ concentrations on rooting and root growth of walnut cotyledon were researched.The results showed that the rooting ability was the worst when the concentration of Fe3＋ was 0 μmol/L,and rooting rate was 66% on the twelfth day.The rooting ability was enhanced markedly when the concentration of Fe3＋ rose,and 0.352 μmol/L was the optimal concentration with 100% rooting rate on the eighth day.Then the cotyledon contacting with medium became to brown when the concentration of Fe3＋ was 0.704 μmol/L.Browning phenomenon was the most seriously with 50% browning rate on the twelfth day when the concentration of Fe3＋ was 7.04 μmol/L,and rooting ability were decreased with 95% rooting rate on the twelfth day.